ID   SYGB_LEUCK              Reviewed;         686 AA.
AC   B1MZ60;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=LCK_00985;
OS   Leuconostoc citreum (strain KM20).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=349519;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KM20;
RX   PubMed=18281406; DOI=10.1128/jb.01862-07;
RA   Kim J.F., Jeong H., Lee J.-S., Choi S.-H., Ha M., Hur C.-G., Kim J.-S.,
RA   Lee S., Park H.-S., Park Y.-H., Oh T.K.;
RT   "Complete genome sequence of Leuconostoc citreum KM20.";
RL   J. Bacteriol. 190:3093-3094(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; DQ489736; ACA82812.1; -; Genomic_DNA.
DR   RefSeq; WP_004909367.1; NC_010471.1.
DR   AlphaFoldDB; B1MZ60; -.
DR   SMR; B1MZ60; -.
DR   STRING; 349519.LCK_00985; -.
DR   EnsemblBacteria; ACA82812; ACA82812; LCK_00985.
DR   KEGG; lci:LCK_00985; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002166; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..686
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101297"
FT   REGION          65..99
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        65..84
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   686 AA;  76496 MW;  AC7C430F288946D6 CRC64;
     MSTFLLEIGL EEVPAHLVTS SESQLVARTK EFLAAHRLTV GDIKPFSTPR RLALQLCDVA
     EESEALSEEK RGPSVERAKD ENGEWSKAAQ GFARGQGATP EAFEERDGYV WLTKHTTGVP
     ANQILSQIGE EVVSQMKFTT YMKWANHSFL YVRPIRWLVA LLDEQVINFN VLDIATGRVT
     RGHRFLSTEH VTISDAQAYE ETLQSAYVLA DAENRKAQIK SQLETIANRN HWVLSLDNAP
     AQDLLEEVNN IVEWPTAFSG SFDQKYLEVP DEVLITSMRE HQRFFYVRDT TGKLLPHFLS
     VRNGDTAHLD NVIAGNEKVL VARLEDAEFF YREDQQKKIA DYMAKVKTLV FHEKIGTVYE
     HMQRVGLLAE KLADALDFDD DKKTDLARAA EIYKFDLMTG MVGEFDELQG VMGEHYARLF
     GENERVATAI REHYMPTSAN GNIAKSDVGA VLAIADKLDA IVTFFAANLI PSGSNDPYGL
     RRAATGVVRT LTTKHWHIAL QPVLAEFMAA TGTVTASADL MAVLSFVVDR VRKLALDDDI
     RQDIVSAGTD NFATADIVYL TDRIQVLANH AHDNNFREVI SALTRVARLA EKTPVSLNVN
     PQLFENETEK ALYAATSELQ LVALEANGAE ALYQALAALQ MPISAYFDAT MVNVDNEQIK
     NNRYAQLNII NQLIAGLGNL EDIVIK