ID   SYGB_LIGS1              Reviewed;         693 AA.
AC   Q1WTP1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=LSL_0911;
OS   Ligilactobacillus salivarius (strain UCC118) (Lactobacillus salivarius).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Ligilactobacillus.
OX   NCBI_TaxID=362948;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCC118;
RX   PubMed=16617113; DOI=10.1073/pnas.0511060103;
RA   Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P.,
RA   Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C., Collins J.K.,
RA   Higgins D., Shanahan F., Fitzgerald G.F., van Sinderen D., O'Toole P.W.;
RT   "Multireplicon genome architecture of Lactobacillus salivarius.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:6718-6723(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000233; ABD99721.1; -; Genomic_DNA.
DR   RefSeq; WP_011476035.1; NC_007929.1.
DR   RefSeq; YP_535804.1; NC_007929.1.
DR   AlphaFoldDB; Q1WTP1; -.
DR   SMR; Q1WTP1; -.
DR   STRING; 362948.LSL_0911; -.
DR   EnsemblBacteria; ABD99721; ABD99721; LSL_0911.
DR   KEGG; lsl:LSL_0911; -.
DR   PATRIC; fig|362948.14.peg.988; -.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000006559; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..693
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006375"
SQ   SEQUENCE   693 AA;  78206 MW;  CD7E92D3B49F3F8D CRC64;
     MAHTFLLEIG LEEIPAHVVT PSVNQLVQKT TKFLKEQRID FDEVIPYSTP RRLTVKVTGL
     ADKQADIEEE AKGPSKKIAL DDEGNWSKAA QGFVRGQGVT VDDIFFKELK GTEYVYVKKF
     IPGKPVSEVL TGMKDVAMDL KFPTMMRWGS NDFEYVRPIK WLVALLDDEV VPFEILDIKT
     GRTTQGHRFL GEAVDVPSAD KYLETLETQK VIADAGVRKA EIRKQIDDLA TENNWNIVVD
     EDLLEEVNNL VEYPTVFAGK FKEEYLQVPN EVLITSMKDH QRFFYVTDKD GNLLPNFVSV
     RNGNKDYLEN VVAGNEKVLT ARLEDAKFFY EEDQQHTIAD YVERLKKVMF HDKIGTIYEK
     MERVNLLAKF LGNKLGLSET ELKDLDRASM IYKFDLVTGM VGEFSELQGI MGEIYARLQG
     EDDNVSTAIR EEYMPTSSEG ELPQSNVGAV LSIADKLDSI QSFFAANMIP SGSNDPYALR
     RQALGIIRIA LDKGWDISLP LLHEAINYAY AEREDLYKNT QPITNVSETD SFVIDRLAQV
     LSGNKFRRDI LDAVVARADM PFIQALQAAQ VLSKHAEDDN FKEVIEALTR VTRLAKKAPE
     FGSDAVIDST LFENDTEKVL ADEFAKVEAG YGDAEMNEKF TILSSLKDSI TAYFDATMIM
     ADDEKVKNNR LLQLVKIAEL TEDFGSLDKL IVK