ID   SYGB_LISW6              Reviewed;         688 AA.
AC   A0AIQ9;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2006, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=lwe1473;
OS   Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / CIP 8149 /
OS   NCTC 11857 / SLCC 5334 / V8).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=386043;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35897 / DSM 20650 / CIP 8149 / NCTC 11857 / SLCC 5334 / V8;
RX   PubMed=16936040; DOI=10.1128/jb.00758-06;
RA   Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S.,
RA   Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O.,
RA   Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T.,
RA   Lampidis R., Kreft J., Goebel W., Chakraborty T.;
RT   "Whole-genome sequence of Listeria welshimeri reveals common steps in
RT   genome reduction with Listeria innocua as compared to Listeria
RT   monocytogenes.";
RL   J. Bacteriol. 188:7405-7415(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AM263198; CAK20891.1; -; Genomic_DNA.
DR   RefSeq; WP_011702266.1; NC_008555.1.
DR   AlphaFoldDB; A0AIQ9; -.
DR   SMR; A0AIQ9; -.
DR   STRING; 386043.lwe1473; -.
DR   EnsemblBacteria; CAK20891; CAK20891; lwe1473.
DR   GeneID; 61189349; -.
DR   KEGG; lwe:lwe1473; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000779; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..688
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006377"
SQ   SEQUENCE   688 AA;  78648 MW;  773D944BB6C4761A CRC64;
     MSKDFLLEIG LEEMPAQYVT SSVAQLEKRV SDWLNENQIT FEKIKTYSTP RRLTVLVEAM
     AEEQANRVEE AKGPAKKIAL DDEGNWSKAA LGFARSQKVE PADLTFREIK GVEYIYIKKE
     VIGEKTTALL PSLEKVVTSM TFPVSMHWGS NDLRYIRPIK WLIAMFGEEI IPFEITGVST
     SNTSRGHRFL GKTATIKQPS DYPNALLEQF VVVNAEERKQ AIVEQLRELE SMENWQIKED
     EDLLEEVTNL VEYPTVLAGN FEKEYLELPE EVLITTMKEH QRYFPVFSKD EELLPHFVTV
     RNGNHENLDT VARGNEKVLR ARLSDADFFY QEDLKITIDE AVAKLQNIVF HEKLGTLTEK
     MKRVQKVALM LADYLDWQEE DKQDIIRLTN IYKFDLVTNI VGEFPELQGL MGEKYALLQG
     EKPAIAIAIR EHYLPSSAEG ALPQTDLGSI IAIADKLETL VGFFCVNIVP TGSADPFGLR
     RSAFGAMRII QANGWDIPVL ELLSRIVDME RAEGAVELPS DDVKKEVQTF LKNRLRVVLQ
     NHHIRHDIID AVIGGDPNTI PQLVDRAQIL NKHVESDWFR PTVEALTRVM NISKKHEGNV
     EVDPSLFENK YEQALFDEIE KLKYDYANLT IVDRLRAFAA LRTTIDDYFD NTLVMSENIE
     LKNNRLALLF ELASFIKEFA QMDEINVK