ID   SYGB_METC4              Reviewed;         696 AA.
AC   B7KUK7;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Mchl_3416;
OS   Methylorubrum extorquens (strain CM4 / NCIMB 13688) (Methylobacterium
OS   extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=440085;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CM4 / NCIMB 13688;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C.,
RA   Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA   Marx C., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium chloromethanicum
RT   CM4.";
RL   Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001298; ACK84236.1; -; Genomic_DNA.
DR   RefSeq; WP_015951543.1; NC_011757.1.
DR   AlphaFoldDB; B7KUK7; -.
DR   SMR; B7KUK7; -.
DR   PRIDE; B7KUK7; -.
DR   EnsemblBacteria; ACK84236; ACK84236; Mchl_3416.
DR   KEGG; mch:Mchl_3416; -.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   BioCyc; MEXT440085:MCHL_RS16615-MON; -.
DR   Proteomes; UP000002385; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..696
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197207"
SQ   SEQUENCE   696 AA;  75671 MW;  DEAB910222B2E29E CRC64;
     MPDLLLELRS EEIPARMQRR AAEDLKKLVT DALVERGFLY EGAKAFATPR RLALHVAGLP
     ARGEAVREER RGPRVGAPEA AVQGFLKSAG LASLDQATTV TDPKKGEFYL AVIERPGRET
     LDVLAEILPG IIKSFPWPKS MRWGAASAQP GSLRWVRPLQ SIVATFGPET ETPEVVPFSV
     DGITAGMVTS GHRFLAPEPF EVRRFDDYVQ ALERADVILD ADRRKDIILH DAKDLAFARG
     LDLVEDEGLL EEVAGLVERP VVLMGSFEER FLEIPAEAIR ATIRANQKCF VLRKSGSDEL
     APAFVLVSNL IASDGGAAIT AGNERVVRAR LSDARFFWET DKATKLEDRL PKLDSIVFHE
     KLGTQGERVA RIAALAAEIA PLVAADPALA ERAARLAKAD LVTEMVGEFP ELQGLMGRKY
     AALQGEHDSV AAAIEEHYKP VGPSDRVPTD PVSIAVALAD KLDTLVGFWA IDEKPTGSKD
     PYALRRAALG VIRAVIERSL RLSLASLLKG RFVSGSDERT ADLLAFFADR LKVYLRDQGA
     RHDLIDAVFA LPGQDDLLMV VRRVEALGAF LATDDGKNLL AGYKRAANIL RIEEKKDGRA
     YDEAPDAALA ASGQPEERAL AEALAAARQE ASAAVAAEDF AGAMRALSRL RAPVDAFFEK
     VTVNADDPAL RKNRLLLLNA LRAATREVAD FSRIEG