ID   SYGB_METFK              Reviewed;         708 AA.
AC   Q1H3M3;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2006, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Mfla_0644;
OS   Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Methylophilaceae; Methylobacillus.
OX   NCBI_TaxID=265072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KT / ATCC 51484 / DSM 6875;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.;
RT   "Complete sequence of Methylobacillus flagellatus KT.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000284; ABE48914.1; -; Genomic_DNA.
DR   RefSeq; WP_011479011.1; NC_007947.1.
DR   AlphaFoldDB; Q1H3M3; -.
DR   SMR; Q1H3M3; -.
DR   STRING; 265072.Mfla_0644; -.
DR   PRIDE; Q1H3M3; -.
DR   EnsemblBacteria; ABE48914; ABE48914; Mfla_0644.
DR   KEGG; mfa:Mfla_0644; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002440; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..708
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101300"
SQ   SEQUENCE   708 AA;  76943 MW;  F29BBFAC25B2AD6F CRC64;
     MSNLLVELFV EELPPKALQN IGISFADQIY HSLVTQDLIE PPSELDAANP SPWKWFASPR
     RIAVWIRNVT QKAPDKQLAQ KLMPASVGLD ADGNATPALL KKLAALGADE SAVSQLKREH
     DGKAEVLLLD RTVRGATLAE GLQKALDEAI AKLPIPKVMT YQLEDGWSNV NFVRPAHGLV
     ALHGNEVVPI RALGLTAGNR TQGHRFEATQ SPIVLRNADS YAEQLQQDGA VIPGFKERRD
     EIARQLEAAA GKLKLRPIED EALLEEVTAL VERPNVLVGQ FEQAFLAVPQ ECLILTMKAN
     QKYFPLLDAQ GRLTNKFLIV SNISPADPSA VVGGNERVVR PRLADAKFFF EQDRKKTLES
     RIPGLSKVVY HNKLGSQGER IARVSEIANL VGTALGDQTL AAQASQAAQL AKADLVTDMV
     GEFPELQGIM GRYYAQHEGL SDDIAYAIED HYRPRFSGDA LPRNKVGMIV ALADKLETLA
     GLFSIGEKPT GEKDPFALRR HAIGILRILT EGGLSLPLNT LLEQTLSVFQ HEFDHDTALQ
     AVEEFIFDRL SGSLRDQGYS VQEVDAVLAM KPQLLGDITK RLEAVRAFAS LPEAASLAAA
     NKRVGNILKK SDSAIEAAIN PALLQEDAEK TLAQALAQVQ PQAQQAFAAG DYTGSLKALA
     ALKAPVDAFF DHVMVNAEDP ALKTNRLALL ATLHQAMNQV ADLSRLAA