ID   SYGB_METNO              Reviewed;         701 AA.
AC   B8IBI6;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Mnod_4369;
OS   Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=460265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J.,
RA   Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium nodulans ORS 2060.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP001349; ACL59240.1; -; Genomic_DNA.
DR   RefSeq; WP_015930881.1; NC_011894.1.
DR   AlphaFoldDB; B8IBI6; -.
DR   SMR; B8IBI6; -.
DR   STRING; 460265.Mnod_4369; -.
DR   EnsemblBacteria; ACL59240; ACL59240; Mnod_4369.
DR   KEGG; mno:Mnod_4369; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000008207; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..701
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197208"
SQ   SEQUENCE   701 AA;  76194 MW;  4F5B31708FED87DC CRC64;
     MPDLLLELFS EEIPARMQRR AAEDLRKLVT DALVERGFLY EGAKAFATPR RLALHIAGLP
     TRGKDVREER KGPRVGAPEA AIQGFLKSAG LTSLADATVV ADPKKGEFYV AVIERPGRDT
     IDVLAEILPA VIRNFPWPKS MRWGAASTEP GSLRWVRPLH SIVASFGPET ETPERVPFAI
     DGIEAGFTTY GHRFLAPGPI EVRRFADYLP ALERARVVLD ADRRRDIILH DARDLAFARG
     LDLVEDEGLL EEVAGLVEWP VTLMGSFDES FLDIPPEVIR ATIRANQKCF VLRAPSQDGR
     ERLANAFILV SNLAASDGGA AIVAGNERVV RARLSDAKFF WETDKKVPLE SRLPKLKDIV
     FHEKLGTQGA RVERIAALAR NLAPLVGADA GLAGRAARLA KADLVTEMVG EFPELQGLMG
     RYYAELQGEP AAIATAIEEH YKPLGPSDRV PTEPVSVAVA LADKLDTLVG FWAIDEKPTG
     SKDPFALRRA ALGVIRLVLA GDVRLPLLGA FAQAVAGHAA VGPESFAADL LGFFADRLKV
     HLRDQGARHD LIDAVFAIPG QDDLLMIVRR VEALGRFLDT EDGRNLLAGF RRAANILRIE
     EKRDGRAYDA APDPALFSLP EESALANALD AARARAAEAV AAEDFEGAMR ALATLRPPVD
     AFFDKVTVNA EDAALRANRL ALLNALREAT RAVADFARIE G