ID   SYGB_METRJ              Reviewed;         699 AA.
AC   B1M1W2;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=Mrad2831_1138;
OS   Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 /
OS   NBRC 15690 / NCIMB 10815 / 0-1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=426355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Marx C.J., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium radiotolerans JCM
RT   2831.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001001; ACB23147.1; -; Genomic_DNA.
DR   RefSeq; WP_012318137.1; NC_010505.1.
DR   AlphaFoldDB; B1M1W2; -.
DR   SMR; B1M1W2; -.
DR   STRING; 426355.Mrad2831_1138; -.
DR   EnsemblBacteria; ACB23147; ACB23147; Mrad2831_1138.
DR   KEGG; mrd:Mrad2831_1138; -.
DR   PATRIC; fig|426355.14.peg.1182; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000006589; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..699
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101301"
SQ   SEQUENCE   699 AA;  75915 MW;  E225BF8641290381 CRC64;
     MPDLLLELRS EEIPARMQRR AAEDLKKLVT DALVARGFLY EGARAFSTPR RLALHIAGLP
     ARGEAVREER RGPRVGAPEA AVQGFLKGAG LASLDQATTV TDPKKGEFYL AVIERPGRET
     LDVLAEFLPE IVRSFPWPKS MRWGAASAEP GSLRWVRPLQ SIVATFGPET ETPEVIPVSV
     GGIAAGTVTY GHRFLAPEAI EVRRFDDYIQ ALERAKVVLD ADRRKDVILH DARDLAFARG
     LDLVEDEGLL EEVSGLVEWP VVLMGSFDES FLDIPAEAIR ATIRANQKCF VLRKGGSEDL
     APAFILVSNL VATDGGQAIT AGNERVVRAR LSDAKFFWET DKATRLEDRL PKLDAIVFHE
     KLGTQGERIA RIAALAKDLA PRVGADPALA ERAARLAKAD LVTEMVGEFP ELQGLMGRKY
     AALQGEHDSV CAAIEEHYKP LGPSDRVPTD PVSIAVALAD KLDTLAGFWA IDEKPTGSKD
     PFALRRAALG VIRLILDRGL RLRLLEQVGA ADRSLAGRAG ADARDLLGFF ADRLKVYLRD
     QGARHDLIDA VFALPGQDDL LMVVRRVEAL AAFLDTEDGK NLLAGYKRAA NILRIEEKKD
     GRAYDAAPDA ALAAAGEPAE RALAEALAKA RETASAAVAR EDFSGAMQAL STLRAPVDAF
     FQDVTVNAPD PKLRENRLAL LNALRAATRE VAEFAKIEG