ID   SYGB_MOOTA              Reviewed;         694 AA.
AC   Q2RKV5;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Moth_0604;
OS   Moorella thermoacetica (strain ATCC 39073 / JCM 9320).
OC   Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC   Thermoanaerobacteraceae; Moorella group; Moorella.
OX   NCBI_TaxID=264732;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39073 / JCM 9320;
RX   PubMed=18631365; DOI=10.1111/j.1462-2920.2008.01679.x;
RA   Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C.,
RA   Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W.;
RT   "The complete genome sequence of Moorella thermoacetica (f. Clostridium
RT   thermoaceticum).";
RL   Environ. Microbiol. 10:2550-2573(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000232; ABC18934.1; -; Genomic_DNA.
DR   RefSeq; WP_011392141.1; NC_007644.1.
DR   RefSeq; YP_429477.1; NC_007644.1.
DR   AlphaFoldDB; Q2RKV5; -.
DR   SMR; Q2RKV5; -.
DR   STRING; 264732.Moth_0604; -.
DR   EnsemblBacteria; ABC18934; ABC18934; Moth_0604.
DR   KEGG; mta:Moth_0604; -.
DR   PATRIC; fig|264732.11.peg.649; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..694
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101303"
SQ   SEQUENCE   694 AA;  77634 MW;  4C7555568E30E7AE CRC64;
     MARDLIFEIG TEEIPARFMN PALEQMAAVA SQLLQEYRLP CQKVATYGTP RRLALYLTAL
     AENQEELVQE IKGPPVKAAF TADGQPTKAA LGFARSMGVA VEELVTREYN GGQYVFAIKR
     EQGRPAVTVL PELLLAVVNA LSFPKPMRWG SLEIRFARPI RWLLALFGSE VIPVELAGLT
     ASNQTYGHRF LAPGPHQVPT ASAYFQVLEE NYVLVDQHRR RQLIWEQITG LASREGGRVE
     KDPELLEEIT YLVEYPTALA GHFDPEYLKL PQEVVITPMR DHQRYFPVWN AQGELLPRFI
     TVHNGTADHL ENISRGNERV LAARLADAAF FYQEDRQTPL AAKVPKLEEI VFQESLGTML
     AKTRRLQRLA VNLVNTLDLP AELVPLVERT AELAKADLVT AMVYEFPELQ GIMGSYYAAH
     DGERAEVCQG IRQHYWPRFA GDRLPDSLTG MVVGLADRLD TLVGCFGAGL IPTGSQDPYA
     LRRQATGVVT IAVEFNLKFS LTASIAAAYE GYTAGGIQLA RPLDVVQEQL VQFCRQRLEH
     LLEEKGYRYD VIQACLAAGS DDLAAAYHRT RDLSAFREEE GFAALQTAFT RAFNLARQAR
     EKYHLRPEAL QEKAEAELYR ALMETRHQAE PRLRAGDYLA ALKAMAALRG PIDAFFDNVL
     VMAPDMEVRN NRLALLQAIV NLVFQIADFS RLVP