ID   SYGB_MYXXD              Reviewed;         703 AA.
AC   Q1D7R9;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=MXAN_3101;
OS   Myxococcus xanthus (strain DK1622).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=246197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DK1622;
RX   PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA   Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA   Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA   Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA   Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT   "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000113; ABF91890.1; -; Genomic_DNA.
DR   RefSeq; WP_011553151.1; NC_008095.1.
DR   AlphaFoldDB; Q1D7R9; -.
DR   SMR; Q1D7R9; -.
DR   STRING; 246197.MXAN_3101; -.
DR   EnsemblBacteria; ABF91890; ABF91890; MXAN_3101.
DR   GeneID; 41360462; -.
DR   KEGG; mxa:MXAN_3101; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_7; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002402; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..703
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101304"
SQ   SEQUENCE   703 AA;  76788 MW;  53004A3AC563432E CRC64;
     MARDLLLEVG AEEIPASFIG PALDDLKRVI TERMADARLK HGEVRTFGTP RRLAVWVKDV
     ADAGEDIIKE VLGPSAKAAF DAQGKPTKAA EKFAESLKLA VDQLGRATTA KGEYLSARVE
     EKGRPAADIL KDTLHAAVHG INFRKSMRWG DVDTSFARPV QWLVALLGSD VLPVVFGDVT
     SGRTTRGHRF LSPDAIELKA PAEYEVALEK AHVVADITKR RAQLVEKVRA AASKAGAQLL
     EDESLVDQVT NLVELPSPVV GTFEERHLDL PPEVLVQEMK SHQRYFSLVD SAGKLQPKFI
     AVSNTPVRDE QLSLRGYQRV LRARLADGRF FFDEDRKTPL IDRVEKLGRV VWQGQLGSYL
     EKVERFRTLA VWLGQEAGRA GEAATIERAA TLAKADLVTG MVGEFPELQG IMGREYARAG
     GEPDAVALAI AEHYLPRGAE DALPTQDPGA LIGIADRLDS LCGIFAIGKA PTGAADPFAL
     RRACIAIIRL VLGRGYRFSL SAAVDESLRL LAPKIANAKR KAGEPAPREQ VLEFFRGRLK
     ALWGEQHRTD VVEAVLSAGF DDLVAAQKRL EALSHIVGRA DFQPLAVAFK RVVNIVEKQG
     RDVQGGETNP QKLVDEPERN LHTAFTQARS TVSGLVRVDD FSGALREITG LKPAVDTFFD
     KVMVMAEDKA LRENRIRLLV EIGALFNQVA DFSKIQAETA AAA