ID   SYGB_NATTJ              Reviewed;         693 AA.
AC   B2A1Y6;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Nther_1208;
OS   Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 /
OS   JW/NM-WN-LF).
OC   Bacteria; Firmicutes; Clostridia; Natranaerobiales; Natranaerobiaceae;
OC   Natranaerobius.
OX   NCBI_TaxID=457570;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C.,
RA   Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J.;
RT   "Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-
RT   LF.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001034; ACB84791.1; -; Genomic_DNA.
DR   RefSeq; WP_012447666.1; NC_010718.1.
DR   AlphaFoldDB; B2A1Y6; -.
DR   SMR; B2A1Y6; -.
DR   STRING; 457570.Nther_1208; -.
DR   EnsemblBacteria; ACB84791; ACB84791; Nther_1208.
DR   KEGG; nth:Nther_1208; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001683; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..693
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101305"
SQ   SEQUENCE   693 AA;  79270 MW;  A01773E69B2CA2A4 CRC64;
     MHNTLLFELG TEELPARFIP GLIKQLEENS ANLLTEYRIE YENIKVMATP RRLTLLVNGL
     ADKQQSSTTM KRGPAKEIAF DENGEPTKAA IGFARGQNID PKDLEIREDN GKEYVYAVAE
     IVGENCQDVL PKLLDELITK FNLPVKMFWN NKQDKFLRPV RWLVCLFNDQ VLPLNFGTVE
     ASNISRGHRF LSQGDVIINS AENYEEVMRD SWVMVDHNVR EETIKAQIEE LADQMNASPE
     MPHDLLEEVN FLVEWPTALL GNFAEEFLEI PKEALITSMQ EHQKYFALVD KEDSSKLLPY
     FVAVRNGDDH GIDKVKAGNE RVLRARLSDA RFFFEEDTKT SLESKREQLK SIIYKEQLGS
     VDQKVTRMEE LGKTLLDILE LSEPVRSLTL RAIALSKADL PTNMVSEFPH LQGIMGEKYA
     RIHGEDEKVC LGISEHYLPR HTGDKLPQTM VGTITSIVDK VDNIASSFAI GERPSGSQDP
     YALRRQALGI VHIILETELA FSLEQLFHRA LSLIPESALV APISNILDDI LDFVKLRSRT
     IFNEQGLPID IIDSTLEVDK ANVYKAYLRA KVLKDYRDSQ ELEDVRTAYT RVKNLANKAK
     GEDVYTELLQ DETEKMLYHK YLNTEDKLLS DLEQDEISSA ISELAGFKDY IDQFFDEVMV
     MVDDKHLQNN RLNLIYHIKE MYRQLADFSI IQD