ID   SYGB_NITEC              Reviewed;         715 AA.
AC   Q0AEY2;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Neut_1868;
OS   Nitrosomonas eutropha (strain DSM 101675 / C91 / Nm57).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=335283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 101675 / C91 / Nm57;
RX   PubMed=17991028; DOI=10.1111/j.1462-2920.2007.01409.x;
RA   Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S.,
RA   Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X.;
RT   "Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas
RT   eutropha C91: implications for niche adaptation.";
RL   Environ. Microbiol. 9:2993-3007(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000450; ABI60100.1; -; Genomic_DNA.
DR   RefSeq; WP_011634903.1; NC_008344.1.
DR   AlphaFoldDB; Q0AEY2; -.
DR   SMR; Q0AEY2; -.
DR   STRING; 335283.Neut_1868; -.
DR   EnsemblBacteria; ABI60100; ABI60100; Neut_1868.
DR   KEGG; net:Neut_1868; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001966; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..715
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101310"
SQ   SEQUENCE   715 AA;  78565 MW;  69F5F554CF63DDAD CRC64;
     MTTESLLIEL LTEELPPKSL VKLGNAFATL IADSLKQQYL TTPDTVSTVF ASPRRLAVHL
     TAISVQAPDQ IVTLKLMPVA VGLDAQGQPT PALHKKLAAL GVENVAVSAL KRVQEGKAEM
     LFLEQSVTGI LLAAGLQKAV DEAIRQLPVA KMMTYQLDDG WENVHFIRPA HGLIALHGQQ
     IVPISALGFA AGNTTRGHRF EAKQAELVID HADHYEYILE TEGTVIPGFD RRRSRIREGL
     ETATRAAQLQ CIEDAALLDE VTALVERPNV LMGTFSADFL EVPQECLIST MKINQKYFPL
     LDADGKLTNR FLIVSNITPA DPGQIIAGNE RVIRSRLADA KFFFDHDRKR TLASRLPDLD
     KVIYHHQLGS QGERTRYVRA LAQIIGQLLD NGDLPAQANQ AAMLAKADLL TDMVGEFPEL
     QGIMGRYYAQ REGVDETIAF AIEDHYKPRF AGDALPRSMT GICVALADKL ETLISLFSIG
     QFPTGDKDPY ALRRHALGVI RILIEKDLPI GLDVLISRAA GVLRDEISGE QGPESSHARP
     VTPQLTEQLQ DFFYDRLASS LRDQGYTVQE VESVLNLRPS LLCEIPRRLA AVRAFTALPE
     AASLAAANKR VSNILKKSEF DATVSIDKAC LQAPAEIALY RALSEIESDA RHAFQKGDYV
     TALQMLAALK APIDAFFDQV MVNDENEVLR RNRLALLAAL QATMNRVADI SRLAA