ID   SYGB_NITEU              Reviewed;         715 AA.
AC   Q82VB0;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=NE1186;
OS   Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC
OS   14298).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=228410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298;
RX   PubMed=12700255; DOI=10.1128/jb.185.9.2759-2773.2003;
RA   Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L.,
RA   Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A.,
RA   Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.;
RT   "Complete genome sequence of the ammonia-oxidizing bacterium and obligate
RT   chemolithoautotroph Nitrosomonas europaea.";
RL   J. Bacteriol. 185:2759-2773(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AL954747; CAD85097.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q82VB0; -.
DR   SMR; Q82VB0; -.
DR   STRING; 228410.NE1186; -.
DR   EnsemblBacteria; CAD85097; CAD85097; NE1186.
DR   KEGG; neu:NE1186; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   PhylomeDB; Q82VB0; -.
DR   Proteomes; UP000001416; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..715
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101311"
SQ   SEQUENCE   715 AA;  78442 MW;  8C6E74D09B053B5E CRC64;
     MMIENLLIEL LTEELPPKSL DKLGNAFAAV IADSLKSQNL TTPDTILTAF ASPRRLAVHL
     TAIPAQAPDQ VVALKLMPIT VGLDAQGQPT PALHKKLAAL GMENVDASAL KRVQESKAEM
     LFLEQNVTGI LLAAGLQKAM EDAIRQLPVS KVMTYQLDDG WENVHFVRPV HGLIALHGQK
     IIPVSAFGLT AGNTTRGHRF EVKQTELIID HADRYASLLE TEGAVIPGFD RRRSWIREGL
     EAAASAVQLR CISDEVLLDE VTALVEYPNI LMGAFPTDFL EVPQECLIST MKINQKYFPL
     LDTDGKLTNQ FLIVANITPA DPGQIISGNE RVIRSRLADA KFFFDHDRKR TLASRLPDLD
     KVIYHHQLGS QGERTRYVQT LARIIGRLLG DDNLAGQADQ AAMLAKADLL TDMVGEFPEL
     QGIMGRYYAR FEGMDETIAF AIEDHYKPRF AGDVLPRSMA GICVALADKL ETLISLFSIG
     QLPTGDKDPY ALRRHALGVI RILIEKNLPI GLDVLISRAA DVLQDEMIGK QDSGPGHARP
     VTPQLVGQLQ DFFYDRLAAS LRDQGYTAQE VEAVLNLRPS LLCEIPRRLA AVRAFAALPE
     AASLAAANKR VGNILKKSEC DATVAIDEAC LQASAEITLY RALSEIESDA RQAFQNGDYV
     TALQILAALK APVDAFFDQV MVNDENEALR RNRLALLMAL QATMNRVADI SRLAA