ID   SYGB_NOSS1              Reviewed;         716 AA.
AC   Q8YPT0;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=alr4111;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; BA000019; BAB75810.1; -; Genomic_DNA.
DR   PIR; AH2319; AH2319.
DR   RefSeq; WP_010998250.1; NZ_RSCN01000010.1.
DR   AlphaFoldDB; Q8YPT0; -.
DR   SMR; Q8YPT0; -.
DR   STRING; 103690.17133246; -.
DR   EnsemblBacteria; BAB75810; BAB75810; BAB75810.
DR   KEGG; ana:alr4111; -.
DR   eggNOG; COG0751; Bacteria.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..716
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_0000072891"
SQ   SEQUENCE   716 AA;  79346 MW;  AC97A4A86E485B9E CRC64;
     MPGFLLEVGT EELPASFLGG AIAQWRSRIP QSLAANSLTS ESVEVYGTPR RLAVLITGLP
     SQQPDREEEI KGPPAQAAFK DGQPTPAAVG FAKKQGVELE ALEVRPTDKG EFVFIQKKTP
     GRPVADILTE LVPQWIYSLE GKRLMRWGDG DVRFSRPIRW LVALLDEAIL PLELVNGSLT
     VKSDRISYGH RVLHPAPITI QKAGDYVNTL RDGYVTVNAK ERTTTIKEQV AATVQKLGGY
     TEIYPDLLEE VTNLVEWPSP VVGKFEEEFL NLPTEVTTTV MVSHQRYFPV FKTGDHKELL
     PYFITVSNGD PTKSEVIAVG NERVIRARLA DGQFFYKTDL SKSLESYLPQ LETVTFQEDL
     GSLRGKVERI VKVAAQISSQ LQLGEGDRQN NQRAALLCKA DLVTQMVFEF PELQGIMGQK
     YAVASGESPE VSTAIVEHYL PRGADDILPQ TLTGQVVGLA DRLDTLVSIF GLGLIPTGSS
     DPFALRRAAN AVVNITWAAN LQINLASLLA QTVAEFAAQY NKDQAPLITA LNEFFLQRIR
     TLLQEEKQID YDLVNAVLGE NDPEYTERAL QDLLDVRDRA LYLQQIRRDG TLDKIYETVN
     RSTRLAAQGD LDTQQLDPTS VVRPELFQKP SESDFYDALV KLVPQTQAAQ ESRNYQLLVA
     GLVEIAPTVS NFFDGADSVL VIDPDPEIKR NRLNILGLLR NHARVLADFG AIVKNL