ID   SYGB_OCEIH              Reviewed;         692 AA.
AC   Q8EPY2;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=OB1948;
OS   Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC
OS   3954 / HTE831).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus.
OX   NCBI_TaxID=221109;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831;
RX   PubMed=12235376; DOI=10.1093/nar/gkf526;
RA   Takami H., Takaki Y., Uchiyama I.;
RT   "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge
RT   and its unexpected adaptive capabilities to extreme environments.";
RL   Nucleic Acids Res. 30:3927-3935(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; BA000028; BAC13904.1; -; Genomic_DNA.
DR   RefSeq; WP_011066345.1; NC_004193.1.
DR   AlphaFoldDB; Q8EPY2; -.
DR   SMR; Q8EPY2; -.
DR   STRING; 221109.22777632; -.
DR   PRIDE; Q8EPY2; -.
DR   EnsemblBacteria; BAC13904; BAC13904; BAC13904.
DR   KEGG; oih:OB1948; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   PhylomeDB; Q8EPY2; -.
DR   Proteomes; UP000000822; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..692
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006380"
SQ   SEQUENCE   692 AA;  79558 MW;  52DAAB4B2E5877F6 CRC64;
     MARDVLIEIG LEELPARFID DAELQLYTKT KQWLEENRIS SENVISYSTP RRLAVFVKNM
     AEKQSSIEED VKGPALKIAK DEDGNWTKAA QGFTKGQGLT TDDIVVREVK GISYIYVTKH
     IEGRPIQELL PEFKSIIESI TFGKNMRWGS ETIRYARPIR WLVAMMGNEV IPFEIAHVAT
     NNVTYGHRFL GEEVTIQEPA EYEKTLKDNY VIVKAKDRET LIVEQLSKLE REHGFEIPVD
     QELLEEVRNL VEFPTAFVGK FEEAYLEIPP EVLITSMKEH QRYFPVHKKG VLQPYFVGVR
     NGDNYHIETV AKGNEKVLRA RLADAEFFYN EDLHQSIDFF QEKLTKVVFQ EKLGTYSDKV
     ERMKQIADRI SEKLSLESDD RKIIERAAEI SKFDLMTSMV NEFTELQGII GEKYANHFGE
     NSATSQAIKE HYQPKHAKDD LPQTVIGSVV SVADKLDTIA GCIAVGLVPT GSQDPYGLRR
     QASAILRILH NEKWNLTVEE LIDIALDVFQ KSNVTIMEKT NEELIEFFRL RAVYLMKDKG
     LEVDVIHAVT DQKLGNVFVS FEKAKELSDK RNDETFKPIQ EALVRVLNLS NKVETNELIR
     EDKLETESEK ILYTRYLDIK ETYEKQLLHN ETKQALATLA QLAAPIHAFF DNNMVMADDL
     EIRNNRLALI QALTSLILPY ADLRKIEWKQ QF