ID   SYGB_OENOB              Reviewed;         689 AA.
AC   Q04F69;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=OEOE_0994;
OS   Oenococcus oeni (strain ATCC BAA-331 / PSU-1).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Oenococcus.
OX   NCBI_TaxID=203123;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-331 / PSU-1;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000411; ABJ56903.1; -; Genomic_DNA.
DR   RefSeq; WP_011677599.1; NC_008528.1.
DR   AlphaFoldDB; Q04F69; -.
DR   SMR; Q04F69; -.
DR   STRING; 203123.OEOE_0994; -.
DR   DNASU; 4415701; -.
DR   EnsemblBacteria; ABJ56903; ABJ56903; OEOE_0994.
DR   KEGG; ooe:OEOE_0994; -.
DR   PATRIC; fig|203123.7.peg.1007; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000774; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..689
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101312"
SQ   SEQUENCE   689 AA;  78628 MW;  3EF0D2C1F477A97E CRC64;
     MADYLLEIGL EEIPAHLVTE SENQLIERIK NFFSDNLLDY KKIQTFSTPR RLAVLVHDLS
     NYSQSKDEEL RGPSLKVAKD ESGNWSRAAE GFARSQGTSP AEFDERDGYV YLNKHIEGVS
     AEEILKKIGI EVVEKMKFST YMKWADFKLE YVRPIRWLVS LLDSKIVPFQ ILDVKADRFT
     RGHRFLSGGK ISISEAGDYE ETLNNAYVIV DAKVRKNSIR NQIRKIADTN DWNLHVDPDL
     LEEVNNIVEY PTAFAGVFDD KYLNLPEIVL TTSMRDNQRF FYVTNKQGKI LPHFISVRNG
     DSNQIENVVK GNEKVLVARL EDAEFFFEED QKHNIDFFMK KAERLVFHEK IGTMTEHMKR
     VEKIAALLAN QLAFNDQEKK DLKRAANICK FDLTTAMVGE FAELQGTMAG IYAKIFGENQ
     TVCQALSEQY LPTSSEGDLP KSKVGAMLAL ADKLDTLFSF FAAGIIPSGS NDPYGLRRAA
     LGIVRIIGQQ KWNFSVSKLL HSLKTAVDKH EDGFLIDFAD TKEISRKVIE FFLDRIRQQS
     SDIRYDLLDA STGKVNEGII NYIFKRVRIL ASHVADPDFR DVIEALTRVQ NLAEKNKSNV
     EIDPELFVTN SEKRLYQLTK DKDPIVLLSK GDHTVYQFLA SLKEPINNYF DENMIMDKNP
     IIKNNRVAQI NLLNNLISSL GDLRKVVVK