ID   SYGB_PERMH              Reviewed;         705 AA.
AC   C0QUJ3;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=PERMA_0569;
OS   Persephonella marina (strain DSM 14350 / EX-H1).
OC   Bacteria; Aquificae; Aquificales; Hydrogenothermaceae; Persephonella.
OX   NCBI_TaxID=123214;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 14350 / EX-H1;
RX   PubMed=19136599; DOI=10.1128/jb.01645-08;
RA   Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S.,
RA   Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A.,
RA   Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A.;
RT   "Complete and draft genome sequences of six members of the Aquificales.";
RL   J. Bacteriol. 191:1992-1993(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001230; ACO03046.1; -; Genomic_DNA.
DR   RefSeq; WP_012675285.1; NC_012440.1.
DR   AlphaFoldDB; C0QUJ3; -.
DR   SMR; C0QUJ3; -.
DR   STRING; 123214.PERMA_0569; -.
DR   PRIDE; C0QUJ3; -.
DR   EnsemblBacteria; ACO03046; ACO03046; PERMA_0569.
DR   KEGG; pmx:PERMA_0569; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_0; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001366; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..705
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197209"
SQ   SEQUENCE   705 AA;  82014 MW;  5D6BD7A871AE7842 CRC64;
     MKKYLLEIGC EELPPKAVDT AISYFKERLE GLFENFFEYR TEENISVFGT PRRIGFILSN
     LREKEPSEEK TILGPPAKVG IDEKGNFTKA ALAFASKNNI PVEQLKIIEN EKGRYIGATL
     IKEGKDIRTF IQEVIPDLIT KIPFPKLMRW NETGFRFSRP VRWIVSLLDN EVVSFSIAGI
     DADRYTHLHR FMTTPTGRGE RKKIPDVDSY FQIMKLGFII PKYEERKEAV KTQLTGFANS
     INAEPVIDED LLDEVTNLTE FPVGILGDFS PEYLILPKEV IITVCKVHQR YFNFEKDGKL
     IPKFLAFSNT AVKDREKVKS GYEKVLKARL EDALFFYEED LKHNLEDFYP QLEGIQFHHK
     LGSMLDKVKR NGEIAVLLSR ELNFENLKDL LRANKLSKCD LLTEMVKEFD ELQGIMGMHY
     ALKQGEKEEV AKAIYEHYLP KTSDDQLPET DIGTLLSLSD KLDTVISFIS IGEKPKATAD
     PFGIRRNSIG IVRILVEKGI DLDLKKLLQD ISREARKVKI LRLADIEKEW EIIFDERTIP
     EILDFIEGRF IAYMKDKGFD TDIINSVVSV DSYNLYRNYL KIKSIQELKK NPEFTDIMTV
     FKRVGRIIPE EFEEHFNPDT LVQDEEKELY RKYTEVNKIF SKDVEDRRYT EALNELLKMK
     PFIDKFFDNV MVMTEDKKLR ENRLSLMKLI NNMFRKIADF TKITT