ID   SYGB_PSEE4              Reviewed;         684 AA.
AC   Q1IH28;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=PSEEN0016;
OS   Pseudomonas entomophila (strain L48).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=384676;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=L48;
RX   PubMed=16699499; DOI=10.1038/nbt1212;
RA   Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C.,
RA   Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P.,
RA   Weissenbach J., Lemaitre B., Medigue C., Boccard F.;
RT   "Complete genome sequence of the entomopathogenic and metabolically
RT   versatile soil bacterium Pseudomonas entomophila.";
RL   Nat. Biotechnol. 24:673-679(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CT573326; CAK13011.1; -; Genomic_DNA.
DR   RefSeq; WP_011531472.1; NC_008027.1.
DR   AlphaFoldDB; Q1IH28; -.
DR   SMR; Q1IH28; -.
DR   STRING; 384676.PSEEN0016; -.
DR   PRIDE; Q1IH28; -.
DR   EnsemblBacteria; CAK13011; CAK13011; PSEEN0016.
DR   KEGG; pen:PSEEN0016; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000658; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..684
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006388"
SQ   SEQUENCE   684 AA;  74936 MW;  3F872B24FAE03FB5 CRC64;
     MSAQDFLVEL GTEELPPKAL ASLGDAFLAG IEKGLQAAGL NYTSKQVYAA PRRLAVLLRQ
     LDVQQPDRSI NIDGPPRQAA FDAEGNPTQA ALGFAKKCGV ELSDIDQSGP KLRFSQHIPG
     KATASLLPTI VEDSLNDLPI PKRMHWGASR EEFVRPTQWL VMLLGDQVVD CTILAQKAGR
     ESRGHRFHHP ENVVITTPAN YVEDLRKAYV LADFAERREL ISKRTAELAM QQEGSAIVPP
     ALLDEVTALV EWPVPLVCSF EERFLEVPQE ALITTMQDNQ KYFCLLDSEG KLLPRFITVA
     NVESRDPKQI VEGNEKVVRP RLTDAEFFFK QDKKQPLESF NERLKNVVFQ AQLGTVFDKA
     ERVSKLAAFI APYIGGSAAN AGRAGLLSKC DLASEMVGEF PEMQGIAGYY YALNDGEPQD
     VALALNEQYM PRGAGAELPQ TLTGAAVAIA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL
     GVLRILIEKQ LDLDLTTAVE FAVKQFGTKV KAAGLSEQVL EFIFDRLRAR YEDEGIDVAT
     YLSVRALKPG SALDFDQRVQ AVQAFRKLPE ANALAAANKR VSNLLGKAEG AIADQVEPKY
     FDNANEFSLY SAIQQADQAV QPMASARQYN EALARLAALR DPVDAFFEAV LVNAEDAKVR
     ANRYALLSRL RGLFLGVADI SLLG