ID   SYGB_PSEF5              Reviewed;         684 AA.
AC   Q4KKR8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=PFL_0013;
OS   Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=220664;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5;
RX   PubMed=15980861; DOI=10.1038/nbt1110;
RA   Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA   Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA   Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J.,
RA   Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C.,
RA   Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III,
RA   Thomashow L.S., Loper J.E.;
RT   "Complete genome sequence of the plant commensal Pseudomonas fluorescens
RT   Pf-5.";
RL   Nat. Biotechnol. 23:873-878(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000076; AAY95431.1; -; Genomic_DNA.
DR   RefSeq; WP_011058403.1; NC_004129.6.
DR   AlphaFoldDB; Q4KKR8; -.
DR   SMR; Q4KKR8; -.
DR   STRING; 220664.PFL_0013; -.
DR   PRIDE; Q4KKR8; -.
DR   EnsemblBacteria; AAY95431; AAY95431; PFL_0013.
DR   KEGG; pfl:PFL_0013; -.
DR   PATRIC; fig|220664.5.peg.13; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000008540; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..684
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006389"
SQ   SEQUENCE   684 AA;  75043 MW;  2E0B18FA358D21C6 CRC64;
     MSAQDFLVEL GTEELPPKAL NTLAEAFLAG IEKGLQSAGL SFQAKHVYAA PRRLAVLITQ
     LATQQPDRSI NLDGPPRQAA FDAEGNPTQA ALGFAKKCGV ELSEIDQSGP KLRYSQSIKG
     KPTASLLPTI VEDSLNDLPI PKRMRWAARK EEFVRPTQWL VMLLGDEVID CTILAQKAGR
     DSRGHRFHHP QSVRITAPAN YLADLRAAYV LADANERREL ISKRTAELAT LQEGTAIVPA
     DLLDEVTALV EWPVPLVCSF EERFLEVPQE ALITTMQDNQ KYFCLLDVDG KLLPRFITVA
     NIESKDPRQI IEGNEKVVRP RLTDAEFFFK QDKKQKLEDF NLRLQNVVFQ EKLGSVYDKA
     ERISKLAAFI APRIGGDAQR AARAGLLSKC DLATEMVGEF PEMQGIAGYY YALNDGEPED
     VALALNEQYM PRGAGAELPS TVTGAAVAIA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL
     GVLRILIEKK FDLDLNDAVA FAVSAFGSKV KAAGLADQVL EFIFDRLRAR YEDEGVDVAT
     YLSVRALKPG SALDFDQRVQ AVQAFRKLPE AAALAAVNKR VSNLLSKIEG NVPTTIEAKY
     FDNANEFSLY SAIQQADQAV QPMAAARQYS ETLARLAALR EPVDAFFEAV MVNAEDAKVR
     ANRYALLARL RGLFLGVADI SVLS