ID   SYGB_PSEFS              Reviewed;         684 AA.
AC   C3KE40;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=PFLU_0010;
OS   Pseudomonas fluorescens (strain SBW25).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=216595;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SBW25;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AM181176; CAY46295.1; -; Genomic_DNA.
DR   RefSeq; WP_012721459.1; NC_012660.1.
DR   AlphaFoldDB; C3KE40; -.
DR   SMR; C3KE40; -.
DR   STRING; 216595.PFLU_0010; -.
DR   PRIDE; C3KE40; -.
DR   EnsemblBacteria; CAY46295; CAY46295; PFLU_0010.
DR   KEGG; pfs:PFLU_0010; -.
DR   PATRIC; fig|216595.4.peg.252; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002332; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..684
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000204609"
SQ   SEQUENCE   684 AA;  74880 MW;  307E9268AC1AFE61 CRC64;
     MSAQDFLVEL GTEELPPKAL NTLADAFLAG IEKGLHSAGL KFEAKKVYAA PRRLAVLLTA
     LETQQPDRSI NLDGPPRQAA FDAEGNPTQA ALGFAKKCGV ELSEIDQSGP KLRFSQVITG
     KPTASLLPTI VEDSLNDLPI PKRMRWGARK EEFVRPTQWL VMLLGDQVID CTLLAQKAGR
     DSRGHRFHHP QAVRITSPAN YAADLRAAYV LADANERREL ISKRTEELAR LQEGTAIVPP
     SLLDEVTALV EWPVPLVCSF EERFLDVPQE ALITTMQDNQ KYFCLLDVDG KLLPRFITVA
     NIESKDPQQI IAGNEKVVRP RLTDAEFFFK QDKKQKLEDF NLRLQNVVFQ EKLGSVYDKA
     VRVSKLAAYI AQRIGGDAAW AARAGLLSKC DLATEMVGEF PEMQGVAGYY YALNDGEPDD
     VALALNEQYM PRGAGAELPT TLTGAAVAIA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL
     GVLRILIDKK LDLDLTQAVV FAVGQFGAKV KQAGLAEQVL EFVFDRLRAR YEDEGVDVSV
     YLSVRALQPG SALDFDQRVQ AVQAFRKLPE ADALASVNKR VSNLLSKAEG LGNADVDPGL
     FADAKEFSLN SAIAKAENAV KPLIAERNYA EALARLATLR EPVDAFFEAV MINAEDAGVR
     KNRYAMLARL RGLFVNIADI STLS