ID   SYGB_PSEP1              Reviewed;         684 AA.
AC   A5VWJ1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Pput_0076;
OS   Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=351746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT   "Complete sequence of Pseudomonas putida F1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000712; ABQ76251.1; -; Genomic_DNA.
DR   RefSeq; WP_011953119.1; NC_009512.1.
DR   AlphaFoldDB; A5VWJ1; -.
DR   SMR; A5VWJ1; -.
DR   STRING; 351746.Pput_0076; -.
DR   EnsemblBacteria; ABQ76251; ABQ76251; Pput_0076.
DR   KEGG; ppf:Pput_0076; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..684
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006392"
SQ   SEQUENCE   684 AA;  75108 MW;  4C2B9CCD647658B6 CRC64;
     MSAQDFLVEL GTEELPPKAL TSLGEAFLAG IEKGLQAAGL NYTGKQVYAA PRRLAVLIRQ
     LDVQQPDRSI NIDGPPLQAA FNAEGEPTQA ALGFAKKCGV ELAEIDQSGP KLRFSQHIPG
     KATTDLLPTI VEDSLNDLPI PKRMRWAASR EEFVRPTQWL VMLLGDQVVD CTILSQKAGR
     ESRGHRFHHP ETVVITTPAN YVEDLRKAHV LADFAERREL ISKRTAELAM QQEGTAIVPP
     ALLDEVTALV EWPVPLVCSF EERFLEVPQE ALITTMQDNQ KYFCLLDSEG KLLPRFITVA
     NIESRDPKQI VQGNEKVVRP RLTDAEFFFK QDKKQPLETF NERLKNVVFQ AQLGTVYDKA
     ERVSKLAAFV APLIGGDAQR AGRAGLLSKC DLATEMVGEF PEMQGVAGYY YALNDGEPED
     VALALNEQYM PRGAGAELPQ TLTGAAVAIA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL
     GVLRILIEKQ LDLDLTGAVE FAVKQFGAKV KAPGLADQVL EFIFDRLRAR YEDEGIDVAT
     YLSVRALQPG SALDFDQRVQ AVQAFRKLPE AEALAAVNKR VSNLLSKAEG AIAEQVEPKY
     FDNANEFSLY SAIQQADQAV QPMAAARQYS ESLARLAALR DPVDAFFEAV MVNAEDAKVR
     ANRYALLSRL RGLFLGVADI SLLG