ID   SYGB_PSEPK              Reviewed;         684 AA.
AC   Q88RR9;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=PP_0060;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AE015451; AAN65694.1; -; Genomic_DNA.
DR   RefSeq; NP_742230.1; NC_002947.4.
DR   RefSeq; WP_010951468.1; NC_002947.4.
DR   AlphaFoldDB; Q88RR9; -.
DR   SMR; Q88RR9; -.
DR   STRING; 160488.PP_0060; -.
DR   PRIDE; Q88RR9; -.
DR   EnsemblBacteria; AAN65694; AAN65694; PP_0060.
DR   KEGG; ppu:PP_0060; -.
DR   PATRIC; fig|160488.4.peg.65; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   PhylomeDB; Q88RR9; -.
DR   BioCyc; PPUT160488:G1G01-63-MON; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..684
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_0000072919"
SQ   SEQUENCE   684 AA;  75113 MW;  C6E5561B2794C599 CRC64;
     MSAQDFLVEL GTEELPPKAL ASLGDAFLAG IEKGLQAAGL NYTGKQVYAA PRRLAVLIRQ
     LDVQQPDRSI NIDGPPLQAA FNAEGEPTQA ALGFAKKCGV ELAEIDQSGP KLRFSQHIPG
     KATVGLLPTI VEDSLNDLPI PKRMRWAASR EEFVRPTQWL VMLLGDQVVD CTILSQKAGR
     ESRGHRFHHP ENVLITTPAN YVEDLRKAYV LADFAERREL ISKRTAELAM QQEGTAIVPP
     ALLDEVTALV EWPVPLVCSF EERFLEVPQE ALITTMQDNQ KYFCLLDSEG KLLPRFITVA
     NVESRDPKQI VQGNEKVVRP RLTDAEFFFK QDKKQPLETF NERLKNVVFQ AQLGTVYDKA
     ERVSRLAAFI APLIGGDAQR AGRAGLLSKC DLATEMVGEF PEMQGVAGYY YALNDGEPED
     VALALNEQYM PRGAGAELPQ TLTGAAVAIA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL
     GVLRILIEKQ LDLDLTGAVE FAVKQFGAKI KAPGLAEQVL EFIFDRLRAR YEDEGIDVAT
     YLSVRALQPG SALDFDQRVQ AVQAFRKLPE AEALAAVNKR VSNLLSKAEG AIAEQVEPKY
     FDNANEFSLY SAIQQADQAV QPMAAARQYS ESLARLAALR DPVDAFFEAV MVNAEDAKVR
     ANRYALLSRL RGLFLGVADI SLLG