ID   SYGB_PSEPW              Reviewed;         683 AA.
AC   B1J425;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=PputW619_0079;
OS   Pseudomonas putida (strain W619).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=390235;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=W619;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S.,
RA   Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Kim E., Taghavi S., Vangronsveld D., van der Lelie D.,
RA   Richardson P.;
RT   "Complete sequence of Pseudomonas putida W619.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000949; ACA70585.1; -; Genomic_DNA.
DR   RefSeq; WP_012312031.1; NC_010501.1.
DR   AlphaFoldDB; B1J425; -.
DR   SMR; B1J425; -.
DR   STRING; 390235.PputW619_0079; -.
DR   PRIDE; B1J425; -.
DR   EnsemblBacteria; ACA70585; ACA70585; PputW619_0079.
DR   KEGG; ppw:PputW619_0079; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..683
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101317"
SQ   SEQUENCE   683 AA;  75021 MW;  DBBB31C2E199F160 CRC64;
     MSAQDFLVEL GTEELPPKAL ATLGDAFLAG IEKGLQAAGL NYTGKQVYAA PRRLAVLIRQ
     LDVQQPDRSI NIDGPPLQAA FKDGEPTQAA LGFAKKCGVE LSEIDQSGAK LRFSQHIPGK
     ATASLLPTIV EDSLNDLPIP KRMRWAASRE EFVRPTQWLV MLLGDQVVDC TILSQQAGRE
     SRGHRFHHPQ NVVITTPANY VEDLRKAYVL ADFAERRELI AKRTTELAMQ QEGTAIVPPA
     LLDEVTALVE WPVPLVCSFE ERFLEVPQEA LITTMQDNQK YFCLLDSEGK LLPRFITVAN
     VESRDPKQIV SGNEKVVRPR LTDAEFFFKQ DKKQPLETFN ERLKNVVFQA QLGTVYDKAE
     RVSRLAAYIA PLIGGDAQRA GRAGLLSKCD LATEMVGEFP EMQGVAGYYY ALNDGEPQDV
     ALALNEQYMP RGAGAELPQT LTGAAVAIAD KLDTLVGIFG IGMLPTGSKD PYALRRAALG
     VLRILIEKQL DLDLTPAVEF AVKQFGAKVK AAGLAEQVLE FIFDRLRARY EDEGIDVATY
     LSVRALKPGS ALDFDQRVQA VQAFRKLPEA EALAAVNKRV SNLLSKAEGA IAEQVEPKYF
     DNANEFSLYS AIQQADQAVQ PMAAARQYSE SLARLAALRD PVDAFFEAVM VNAEDAKVRA
     NRYALLSRLR GLFLGVADIS LLG