ID   SYGB_PSESM              Reviewed;         684 AA.
AC   Q88B35;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=PSPTO_0185;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AE016853; AAO53739.1; -; Genomic_DNA.
DR   RefSeq; NP_790044.1; NC_004578.1.
DR   RefSeq; WP_011103028.1; NC_004578.1.
DR   AlphaFoldDB; Q88B35; -.
DR   SMR; Q88B35; -.
DR   STRING; 223283.PSPTO_0185; -.
DR   EnsemblBacteria; AAO53739; AAO53739; PSPTO_0185.
DR   GeneID; 1181793; -.
DR   KEGG; pst:PSPTO_0185; -.
DR   PATRIC; fig|223283.9.peg.191; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   PhylomeDB; Q88B35; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..684
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_0000072920"
SQ   SEQUENCE   684 AA;  74773 MW;  021F0DD84F18C482 CRC64;
     MSAQDFLVEL GTEELPPKTL VSLADAFLAG IEKGLAGAGL TYSAKQVYAA PRRLAVLITA
     LATQQPDRSV NLDGPPRQAA FDADGNPTQA ALGFAKKCGV DLSEIDQSGP KLRYSQTILG
     KPTTSLLPTI VEDSLNDLPI AKRMRWGARK EEFVRPTQWL VMLFGDQVVD CTILAQSAGR
     HSRGHRFHHP EDVRISSPAG YLSDLRAANV LADFNERRQI ISKRVEELAS QQEGTAIVPP
     SLLDEVAGLV EWPVPLVCSF EERFLDVPQE ALITTMQDNQ KYFCLLDADG KLLPRFITVA
     NIESKDPAQI IAGNEKVVRP RLTDAEFFFK QDKKQKLETF NDRLKNVVFQ AQLGSVFDKA
     ERVSKLAAYI APRIGGDAQR AARAGLLSKC DLSSEMVGEF PEMQGIAGYY YAKADGEAED
     VALALNEQYM PRGAGAELPT TLTGAAVAIA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL
     GILRILIEKK LDLNLVETVK FAVTQFGAKV KPAGLAEQVL EFIFDRLRAR YEDEGVEVAV
     YLSVRALQPA SALDFDQRVQ AVQAFRKLPQ AAALAAVNKR VSNLLSKAEG SIAQTVEPKY
     FDNANEFSLY SAIQQADHAV QPMAAERQYS ESLARLAMLR EPVDAFFEAV MVNAEDANVR
     ANRYALLARL RGLFLGVADI SLLG