ID   SYGB_PSET1              Reviewed;         689 AA.
AC   Q3IJ28;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=PSHAa0006;
OS   Pseudoalteromonas translucida (strain TAC 125).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Pseudoalteromonadaceae; Pseudoalteromonas.
OX   NCBI_TaxID=326442;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TAC 125;
RX   PubMed=16169927; DOI=10.1101/gr.4126905;
RA   Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N.,
RA   Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C.,
RA   Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C., Rouy Z.,
RA   Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A.;
RT   "Coping with cold: the genome of the versatile marine Antarctica bacterium
RT   Pseudoalteromonas haloplanktis TAC125.";
RL   Genome Res. 15:1325-1335(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CR954246; CAI85120.1; -; Genomic_DNA.
DR   RefSeq; WP_011326738.1; NC_007481.1.
DR   AlphaFoldDB; Q3IJ28; -.
DR   SMR; Q3IJ28; -.
DR   STRING; 326442.PSHAa0006; -.
DR   EnsemblBacteria; CAI85120; CAI85120; PSHAa0006.
DR   KEGG; pha:PSHAa0006; -.
DR   PATRIC; fig|326442.8.peg.6; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   BioCyc; PHAL326442:PSHA_RS00030-MON; -.
DR   Proteomes; UP000006843; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..689
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006390"
SQ   SEQUENCE   689 AA;  75889 MW;  313A5758BC9367D8 CRC64;
     MSAQNLLVEI GTEELPPKAL RKLAEAFAAN LTAELETLEL AHQGVSWYAS PRRLGLRVIA
     LDAKQQDKEV EKRGPAIKAA FDADGNPTKA AMGWARGCGI EVKDAQTLET DKGAWLLHLA
     KVAGQETKTL MVDVINKALA KLPIPKPMRW GANKTQFIRP VHTVTILLGD ELVTGEVLGK
     QVSNQLQGHR FHHPEKITIN HADDIFDVLK SAYVIADYEQ RKAQIRAQIE AAAKAINAVV
     AMDEDLLEEV TSLVEWPVTL TATFEEEFLA VPAEALIYTM KDDQKYFPLL DQNGKLLNKF
     LFVSNIESKD PSVVISGNEK VVRPRLADAQ FFFESDKKKT LESRLESLDS VLFQKQLGTL
     KDKSARISEL AGYIAQQLGA DKELAQRAGL LSKTDLMTEM VMEFTDIQGV MGMHYARFDG
     EAEDVALAQN EQYMPRFAGD SLPTNLISCA VAIADKFDTL VGIFGIGQAP KGDKDPFALR
     RAAIGALRIM VEKELPLDIL DLVAKSQTLF GDKLTNLNVS TDVFEFMLGR FRAWYQDEGI
     EVDVIQAVLA RRPTKPVDFD RRVKAVSHFR TLDAAEALAA ANKRVSNILA KNDITSEGNV
     DESLLTDDAE KVLAAQVAKF ATELAPLYAS GNYQEALSQL AGIRQSVDSF FDNVMVMADD
     EAVKQNRLAL LSQLSGLFLG IADISVLQK