ID   SYGB_PSEU5              Reviewed;         684 AA.
AC   A4VFG6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=PST_0009;
OS   Pseudomonas stutzeri (strain A1501).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=379731;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A1501;
RX   PubMed=18495935; DOI=10.1073/pnas.0801093105;
RA   Yan Y., Yang J., Dou Y., Chen M., Ping S., Peng J., Lu W., Zhang W.,
RA   Yao Z., Li H., Liu W., He S., Geng L., Zhang X., Yang F., Yu H., Zhan Y.,
RA   Li D., Lin Z., Wang Y., Elmerich C., Lin M., Jin Q.;
RT   "Nitrogen fixation island and rhizosphere competence traits in the genome
RT   of root-associated Pseudomonas stutzeri A1501.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:7564-7569(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000304; ABP77717.1; -; Genomic_DNA.
DR   RefSeq; WP_011911260.1; NC_009434.1.
DR   AlphaFoldDB; A4VFG6; -.
DR   SMR; A4VFG6; -.
DR   STRING; 379731.PST_0009; -.
DR   PRIDE; A4VFG6; -.
DR   EnsemblBacteria; ABP77717; ABP77717; PST_0009.
DR   KEGG; psa:PST_0009; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000000233; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..684
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006395"
SQ   SEQUENCE   684 AA;  74904 MW;  55DA058276D83A81 CRC64;
     MSALDFLVEL GTEELPPKAL AKLADAFCAG IEKGLKDAGL GFAKAQAYAA PRRLAVLVEQ
     LATQQPDRSI NLDGPPMQAA FDAHGEPTQA ALGFARKCGV DLAEIDRSGP KLKFSRTIEG
     QPATQLLPGI VEASLNDLPI PKRMRWAARK EEFVRPTQWL VMLFGEQVID CEILAQRAGR
     ESRGHRFHSP GQVHISKPSS YLEDLRGAHV IADFAERREL IAKRVEQLAS EQNGSAIVPP
     ALLDEVTALV EWPVPLVCSF EERFLEVPQE ALISTMQDNQ KYFCLLDTNG KLLPRFITVA
     NIESKDPAQI VSGNEKVVRP RLTDAEFFFK QDKKQPLERF NDRLKNVVFQ AQLGTVFDKA
     ERVSRLAGLI AERTGGDKAR AMRAGLLSKA DLATEMVGEF PEMQGIAGYY YALNEGEPED
     VALALNEQYM PRGAGGELPS TLTGAAVAVA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL
     GVLRILIEKQ LDLNLVEAVN FAIGQFGTQV KSAGLADQVL EFIFDRLRAR YEDEGVDVAA
     YLSVRAVQPG SALDFDQRVQ AVQAFRTLPE AEALAAANKR VSNLLAKFEA KLPEAVEPRW
     FDNATEFSLY SALQQAEQAV QPLAAARQYR EALERLAHLR GPVDAFFEAV LVNAEDASVR
     ANRYALLARL RGLFLGVADI SALG