ID   SYGB_RALPJ              Reviewed;         697 AA.
AC   B2UFN5;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Rpic_0400;
OS   Ralstonia pickettii (strain 12J).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=402626;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=12J;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C.,
RA   Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Mikhailova N., Marsh T., Richardson P.;
RT   "Complete sequence of chromosome 1 of Ralstonia pickettii 12J.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001068; ACD25558.1; -; Genomic_DNA.
DR   RefSeq; WP_009239103.1; NC_010682.1.
DR   AlphaFoldDB; B2UFN5; -.
DR   SMR; B2UFN5; -.
DR   STRING; 402626.Rpic_0400; -.
DR   EnsemblBacteria; ACD25558; ACD25558; Rpic_0400.
DR   GeneID; 61387990; -.
DR   KEGG; rpi:Rpic_0400; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..697
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101318"
SQ   SEQUENCE   697 AA;  75193 MW;  E1A1F7B569324CC9 CRC64;
     MSTLLIELLT EELPPKALAR LGEAFARGLF DGLSAQGLLE EGAAVEGFAT PRRLAASITG
     ARRTAPDREL REKVLPVTIA FDAEGKPAAP LVKKLAALAK TVGVEVIAAE SLERGPDGKA
     EALFYRYTAR GAVLADGLQT ALSQTIANLP IPKVMTYQRP NGENVQFVRP AHKLIALLDS
     EVIPVSAFGL QSGNVTLGHR FLSAGEIVIQ DAASYASTLE SQGKVIAGYG KRKEAIRAEL
     LKTAGADTVV MPEALLDEVN ALVEWPVVYP CHFEEAFLAV PQECLILTMQ TNQKYFALTD
     AQGHLRNRFL IVSNIATDTP EAIIQGNERV VRPRLADARF FFEQDKKKPL ADRVPLLSRV
     VYHNKIGTQL ERVSRLQAIA GQLAEKLGAD VAHASRGALL AKADLLTDMV GEFPELQGTM
     GTYYARHDGE PDDVALACSE HYQPRFAGDA LPSTATGTVV ALADKLETLV GIWGIGLQPT
     GEKDPFALRR HALGILRMLI EKPLALTIDD ALQIAAASFD GIAAVKPNLA AITDFLYDRL
     RGYLKDKGYS TNEVEAVVSQ RPQRLDDIVA RLEAVRAFAA LPQAEALAAA NKRITNILKK
     TDVAIGAVQP PLLKEDAERA LHQSVVSAEP QVHDAFARGD FTTALKTLAG LREAVDTFFD
     GVMVMADDTA LRDNRLALLA ELHGLMNRVA DISKLAA