ID   SYGB_RHIL3              Reviewed;         704 AA.
AC   Q1MKQ6;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=RL0951;
OS   Rhizobium leguminosarum bv. viciae (strain 3841).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=216596;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3841;
RX   PubMed=16640791; DOI=10.1186/gb-2006-7-4-r34;
RA   Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F.,
RA   Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H.,
RA   East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I.,
RA   Chillingworth T., Clarke K., Cronin A., Davis P., Fraser A., Hance Z.,
RA   Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA   Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J.;
RT   "The genome of Rhizobium leguminosarum has recognizable core and accessory
RT   components.";
RL   Genome Biol. 7:R34.1-R34.20(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AM236080; CAK06448.1; -; Genomic_DNA.
DR   RefSeq; WP_011650690.1; NC_008380.1.
DR   AlphaFoldDB; Q1MKQ6; -.
DR   SMR; Q1MKQ6; -.
DR   STRING; 216596.RL0951; -.
DR   EnsemblBacteria; CAK06448; CAK06448; RL0951.
DR   KEGG; rle:RL0951; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000006575; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..704
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101321"
SQ   SEQUENCE   704 AA;  77225 MW;  7ECCB4836AE2487C CRC64;
     MPNLLLELRS EEIPARMQRK AAGDLKKLVT DALVEAGLSY EGAREYWTPR RLALDIHGLT
     ARSADVREER KGPRTDANEK AIEGFLRGAG LSSVSEAQVV SDPKKGDFYV AVISKPGRAT
     EEIVTDVMPG IIRDFPWPKS MRWGKASSKP GALRWVRPLQ SIVCTFGPEH EETTVIPFEI
     DGITASNITY GHRFHAPEPI TVRRFDDYAA NLEKAKVILD AERRKDIILH DARDIAFANG
     LELVEDEGLL EEVSGLVEWP QVLMGSFEED YLSIPSEIIR LTIKTNQKCF VTRPQGGETL
     SNKFILVSNI QASDGGKEIV HGNGKVVRAR LSDALHFWKR DQGNLPDLET LTASAAKFGL
     DINKPLDQRM AKLDALDVTF HAKLGTQGAR VARIRTLAKQ LADITGADAA LIDRAAVLAK
     ADLRTEAVGE FPELQGLMGR KYAALQGEDA SVAAALEDHY KPQGPSDRVP EDRVAITIAL
     ADKLDTLIGF WAIDEKPTGS KDPFALRRAA LGVVRILLER KVRLPLLTTT RDIDLLSFFH
     DRLKVYLRDQ GARHDLIDAV LTPDADDLLM VARRVEALTA FITSEDGKNL LAGTKRATQL
     LAAEEKKGTV IADGVSQALL KLDAEKELFA AISGASKDAS DAVAGEDFRS AMEALSKLRG
     PVDRFFEDVL VNDEDAAIRA NRLALLRLIR EATGTVADFS KISG