ID   SYGB_RHORT              Reviewed;         721 AA.
AC   Q2RQ43;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Rru_A2955;
OS   Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 /
OS   NCIMB 8255 / S1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Rhodospirillum.
OX   NCBI_TaxID=269796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1;
RX   PubMed=21886856; DOI=10.4056/sigs.1804360;
RA   Munk A.C., Copeland A., Lucas S., Lapidus A., Del Rio T.G., Barry K.,
RA   Detter J.C., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D.,
RA   Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Kyrpides N.C.,
RA   Mavromatis K., Richardson P., Rohde M., Goeker M., Klenk H.P., Zhang Y.,
RA   Roberts G.P., Reslewic S., Schwartz D.C.;
RT   "Complete genome sequence of Rhodospirillum rubrum type strain (S1).";
RL   Stand. Genomic Sci. 4:293-302(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000230; ABC23752.1; -; Genomic_DNA.
DR   RefSeq; WP_011390705.1; NC_007643.1.
DR   RefSeq; YP_428039.1; NC_007643.1.
DR   AlphaFoldDB; Q2RQ43; -.
DR   SMR; Q2RQ43; -.
DR   STRING; 269796.Rru_A2955; -.
DR   EnsemblBacteria; ABC23752; ABC23752; Rru_A2955.
DR   KEGG; rru:Rru_A2955; -.
DR   PATRIC; fig|269796.9.peg.3065; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   PhylomeDB; Q2RQ43; -.
DR   Proteomes; UP000001929; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..721
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101324"
SQ   SEQUENCE   721 AA;  77875 MW;  83D5D2442D77396B CRC64;
     MAELLLELLS EEIPARMQVR AIEDLTKLVG TGLAEAGLSH GGLRGFVTPR RLTLVVDGLP
     VAQPDVHEER RGPKADAPDK AIAGFLGSLG LTRDQLETRE TPKGPFLFAV IERKGRPTAE
     VLPEIIYRAL TALSWPKSMR WARGQFTWVR PLHSILAVFE GAPLRGAIGL GVSLEGLPVG
     FIADPAAAPE GTPLLAFGAT TRGHRFLAPE PFPVRDFADY AARLRQAHVL IDREERKRVI
     AERAAALAAG EGLRVKDDPA LLEEITGLVE WPVPLMGHID KAFMDVPAEV LTTSMRSHQK
     YFSVENADGT LNARFVVVSN MDATDSSARV VAGNERVLRA RLSDAKFFWD QDRKAPLAGK
     VEALGARVFH AKLGSDLDKV TRVRAGAAWL AARVPGADAS LVDRAALLAK ADLTTGMVGE
     FPELQGLMGR YYALHDGEPP AVAQAIRDHY SPVGPGDACP TAATSVCVAL ADKIDTLAGF
     WLIDEKPTGS KDPFALRRAA LGVIRLIVEN SLRLPLREVL GSAISTYGDR MLAQCGKADG
     TSSDVIEHDL IAFIAERLKV QQREKGVRHD LIEAVFSQGG EDDLVRLLAR VSALADFLAS
     EEGTNLLSAY RRATNIVRIE EKKDGKPVSG DPDNALFEQD EERALSLALD TLLGEVSPSL
     AAEDHAAAMR ALARLRGPVD AFFDRVTVNA DTPALRNNRL RLLARIGTAM ATLADFSKIE
     G