ID   SYGB_RICAE              Reviewed;         664 AA.
AC   C3PLY4;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=RAF_ORF1203;
OS   Rickettsia africae (strain ESF-5).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=347255;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ESF-5;
RX   PubMed=19379498; DOI=10.1186/1471-2164-10-166;
RA   Fournier P.-E., El Karkouri K., Leroy Q., Robert C., Giumelli B.,
RA   Renesto P., Socolovschi C., Parola P., Audic S., Raoult D.;
RT   "Analysis of the Rickettsia africae genome reveals that virulence
RT   acquisition in Rickettsia species may be explained by genome reduction.";
RL   BMC Genomics 10:166-166(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001612; ACP53974.1; -; Genomic_DNA.
DR   RefSeq; WP_012720092.1; NC_012633.1.
DR   AlphaFoldDB; C3PLY4; -.
DR   SMR; C3PLY4; -.
DR   EnsemblBacteria; ACP53974; ACP53974; RAF_ORF1203.
DR   KEGG; raf:RAF_ORF1203; -.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000002305; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..664
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000204610"
SQ   SEQUENCE   664 AA;  75601 MW;  7BC976A8CA2046E8 CRC64;
     MSELLLELFS EEMPAFMQKN AEEGYLNIFT KIFEENEIFA KVQVFVGPRR ITLHATHLPK
     ITLPKEEEIK GPSIEAPEAA INGFCKAHNV SKLELPTKLI SNQLYYFFVK KTEEREIKEI
     LPEIIIEAIN KYSWAKSMFW GDYKIKWIRP LRNILCIFDG EILPMQFGHL TANNITYGHR
     LTDNKKLEVT DFEDYRNKLL ENHVILERAK REAIIKTGLL ELANSHELII KEDNRLVEEV
     VGLSEFPVVL LGKIPQKFLE LPKEVLISSM RTHQKYFCLF DKTGNFTPYF LFVSNGRFTN
     AELVIQGNEK VLSARLSDAL YFCKQDIAKT LESRLGQLEA ATFHAKLGNL REKIERITDI
     CNYIAPNNKD LITAARLCKS DLVSEMVGEF PDLQGIMGYY YAKHEGLNAE IAAAIRDHYK
     PQGLSDNLPS GNAALLALAD KLDSLVGLMI AGETPTGSGD PYALRRQALG IIRIILENKL
     ELNFNDLINF SINLYKDSSD ENKNLIISFF KERAKFYFKN DYDIALINAV LDLNLVDTNF
     KLDALKEFLI EDAGKQLLNA YKRASNIIGD QKITGLVDAS LFSTQPEKEL FEVIQKISPQ
     IIDSIADKDY KKALNLLSSL LTPITSFFDN VLVNDSDPKI AQNRLSLLQN ICELFDKVAK
     FNRL