ID   SYGB_RICAH              Reviewed;         662 AA.
AC   A8GQ64;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=A1C_06580;
OS   Rickettsia akari (strain Hartford).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=293614;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Hartford;
RA   Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Dasch G., Eremeeva M.;
RT   "Complete genome sequence of Rickettsia akari.";
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000847; ABV75539.1; -; Genomic_DNA.
DR   RefSeq; WP_012150168.1; NC_009881.1.
DR   AlphaFoldDB; A8GQ64; -.
DR   SMR; A8GQ64; -.
DR   STRING; 293614.A1C_06580; -.
DR   EnsemblBacteria; ABV75539; ABV75539; A1C_06580.
DR   KEGG; rak:A1C_06580; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000006830; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..662
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101327"
SQ   SEQUENCE   662 AA;  75145 MW;  D66ADB0E50F98E34 CRC64;
     MSELLLELFS EEIPAFMQKN AEEGYLNIFT QIFAESAIFA KVQVFVGPRR ITLYATHLPK
     VTLPQEEEIK GPSIESPEAA INGFCSAHNV SKLELSTKLI NNQLYYFFVK KTKEREIKEI
     LPKIIIEAIN KYSWAKSMFW GDYKIKWIRP LRNILCIFDG EVLPLQFGHL TANNITYGHR
     LTDNKKLEVT DFKDYRNKLL ENNVVLERIK REEIIKTGLL ELANSQNLNI KEDARLIEEV
     AGLSEFPVVL LGKIAQKFLE LPKEVLIASM RTHQKYFCLF DKTGNFAPYF LFVSNGRFAN
     AELVIQGNEK VLSARLSDAL YFYKQDIAKT LESRLGKLEA VTFHAKLGNL REKIEHITDI
     CNYIAPNNKD LITAAKLCKS DLVSEMVGEF PDLQGIMGYY YAKYEGLNEE IAAAIRDHYK
     PQGLSDNVAS GNAALLALAD KLDSLVGLMI AGEAPTGSGD PYALRRQALG IIRIILENKL
     ELNLNDLIIF SLKLYGNSAD KDLITSFFEE RAKFYFKNKY DILLINAVLD LNLVDTQFKL
     ETLKEFLIED AGKQLLNAYK RASNIIGDQK ITGLVDASLF STQPEKDLFE VVQKISPQII
     DSIADKDYKR ALNLLSFLLT PITSFFDNVL VNDPDPKIAQ NRLSLLQNIC ELLNKIAKFN
     RL