ID   SYGB_RICCK              Reviewed;         667 AA.
AC   A8F071;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=A1E_05440;
OS   Rickettsia canadensis (strain McKiel).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; belli group.
OX   NCBI_TaxID=293613;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=McKiel;
RA   Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Dasch G., Eremeeva M.;
RT   "Complete genome sequence of Rickettsia canadensis.";
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000409; ABV74004.1; -; Genomic_DNA.
DR   RefSeq; WP_012149199.1; NC_009879.1.
DR   AlphaFoldDB; A8F071; -.
DR   SMR; A8F071; -.
DR   STRING; 293613.A1E_05440; -.
DR   EnsemblBacteria; ABV74004; ABV74004; A1E_05440.
DR   KEGG; rcm:A1E_05440; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000007056; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..667
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101329"
SQ   SEQUENCE   667 AA;  75818 MW;  19BC966D6F78E871 CRC64;
     MSELLLELFS EEIPAFMQKN AEKGYLNVFT KIFEENKIFA KVKVFVGPRR ITLHATHLPK
     VTLPKDTAIK GPSLNASEAA INGFCKAHNV SKLELFTKLI NNQLYYFFIK KTEEREIKEI
     LPEIIIEAIN KYSWTKSMFW GDYNIKWVRP LRNILCIFDG EILPMQFGHL TANNITYGHR
     LTDNKKIEVE NFEDYKNKLS ENHVILERTK REEIIKAGLL ELANSHDLTI KEDNRLIEEV
     SGLTEFPVVL LGRIPLKFLE LPKEVLISSM RRHQKYFCLF DKAGNFAPYF LFVSNGRFVN
     AELVIKGNEK VLAARLADAL YFYKQDIAKT LESNLSKLLA VTFHVKLGNL REKVDRITNI
     CNYIAPNNKD LIMAAKLCKS DLVSEMVGEF PDLQGIMGYY YAKHDGLDEE VATAIKDHYK
     PQGLSDNVPN GNAALLATAD KIDSLVGLMI AGEAPTGSGD PYALRRQALG IIRIIIENKL
     ELNLNDLINF SINLYKDSSD KNKDLIISFL KERIKFYFKN NYDISLINAV LDLSAEDLVS
     VSLKLNTLQK FLAEDAGKQL LNAYKRASNI IGDQKITGLV DGNLFTTQSE KELFAVMQKI
     LPQIIDSITD KDYERVLNLL SSLLAPITSF FGNVLVNDPD PKIVQNRLSL LQNTCELFHK
     IAKFNRL