ID   SYGB_RICFE              Reviewed;         664 AA.
AC   Q4UJU2;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=RF_1346;
OS   Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=315456;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA   Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA   Parinello H., Claverie J.-M., Raoult D.;
RT   "The genome sequence of Rickettsia felis identifies the first putative
RT   conjugative plasmid in an obligate intracellular parasite.";
RL   PLoS Biol. 3:1-12(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000053; AAY62197.1; -; Genomic_DNA.
DR   RefSeq; WP_011271646.1; NC_007109.1.
DR   AlphaFoldDB; Q4UJU2; -.
DR   SMR; Q4UJU2; -.
DR   STRING; 315456.RF_1346; -.
DR   EnsemblBacteria; AAY62197; AAY62197; RF_1346.
DR   KEGG; rfe:RF_1346; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000008548; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..664
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_0000274898"
SQ   SEQUENCE   664 AA;  75293 MW;  90A7C83087E3FD52 CRC64;
     MSELLLELFS EEIPAFMQKN AEEGYLNIFT KIFEENEIFA KVQAFAGPRR ITLYATHLPK
     VTLPKETEIK GPSIDAPEAA INGFCKAHNV SKLELSTKLI NNQLYYFFVK KTEEREIKEI
     LPEIIIEAIN KYSWAKSMFW GDYKIKWIRP LRNILCIFDG EVLPLQFGHL TANNIAYGHR
     YRLTDNKKLE VTDFEDYKNK LSENHVILER TKREEIIKTG LLELANSRNL NIKEDNRLIE
     EVAGLSEFPV VLLGKIPQKF LELPKEVLIS SMRTHQKYFC LFDKEGNFAQ YFLFVSNGRF
     ANAELVIKGN EKVLSARLSD ALYFYKQDIA KTLESRLGKL EAVTFHAKLG NLREKVERVA
     KICSYTALDN TDLITAAKLC KSDLVSEMVG EFPDLQGIMG YYYAKHEGLG EEVAAAIKDH
     YKPQGLSDNV PGGNAALLAL ADKVDSLVGL MIAGEAPTGS GDPYALRRQA LGIIRIILEN
     KLELNLNDLI IFSLKLYGNS ADKDLITSFF EERAKFYFKN DYDIALINAA LDLNLVDTKF
     KLDSLKEFLV EDAGKQLLNA YKRASNIIDG QKITGLVDAS LFSTQPEKEL FEVMQKISPQ
     VTSSISDKDY NKALNLLSFL LTPITSFFDN VLVNDPDPKI AENRLSLLHN ICELFDKVAK
     FCRL