ID   SYGB_RICTY              Reviewed;         664 AA.
AC   Q68VR4;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=RT0838;
OS   Rickettsia typhi (strain ATCC VR-144 / Wilmington).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=257363;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-144 / Wilmington;
RX   PubMed=15317790; DOI=10.1128/jb.186.17.5842-5855.2004;
RA   McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E.,
RA   McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E.,
RA   Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A., Hong C.,
RA   Yu X.-J., Walker D.H., Weinstock G.M.;
RT   "Complete genome sequence of Rickettsia typhi and comparison with sequences
RT   of other Rickettsiae.";
RL   J. Bacteriol. 186:5842-5855(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AE017197; AAU04292.1; -; Genomic_DNA.
DR   RefSeq; WP_011191266.1; NC_006142.1.
DR   AlphaFoldDB; Q68VR4; -.
DR   SMR; Q68VR4; -.
DR   STRING; 257363.RT0838; -.
DR   EnsemblBacteria; AAU04292; AAU04292; RT0838.
DR   KEGG; rty:RT0838; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000604; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..664
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_0000274899"
SQ   SEQUENCE   664 AA;  76205 MW;  B30A6BB6DC1FB006 CRC64;
     MSELLLELFS EEIPAFMQKN AEEAYLNIFT KIFKENEIFA QVQVFVGPRR ITLHATNLPK
     VILPKEEEIK GPSIEAPEIA INGFCKVHNV NKFDLSTKLI NKKLYYFFVK KTQAREMKEI
     LPKIIIDAIN KYSWIKSMFW GCYKIKWIRP LRNILCIFDG EILPLQFGHL SANNITYGHR
     LTNNKKLEII DFENYRNKLL ENNVILERLK REEIIKVGLL ELANAQNLNI KQDARLIEEV
     TGLNEFPVVL LGKIPQKFLE LPEEVIISVM RKHQKYFCLF DKTGNFAPYF LFVINGRFVN
     IELILQGNEK VLSARLADAL YFYKHDIAKT LESRFSKLES VIFHAKLGSL KEKVDRITDI
     CRYIAPDNID LIMAARLCKS DLVSDMVGEF PDLQGIMGYY YAKHEGLNEE VAKAIRDHYK
     PQGLNDNVPS GNATLLALAD KLDSLVGLII VGETPNGSGD PYALRRQALG IIRIIIENKL
     EINFINLINF AVSLYKVPTN THLDSVISFF EERAKFYFKN DYDIALINAV LDLNLVDTKF
     KLDTLKEFLV QDVGKQLLNA YKRVSNIIGN QKITGLVDVS LFSTQYEKEL FEVIQIISQQ
     IIAIIANKDY KKALNLLSSL LKPITSFFDN VLVNDSDPKI AQNRLSLLHN TCEVFDKVVK
     FCRL