ID   SYGB_RUEST              Reviewed;         687 AA.
AC   Q1GE74;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2006, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=TM1040_2310;
OS   Ruegeria sp. (strain TM1040) (Silicibacter sp.).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Roseobacteraceae; Ruegeria; unclassified Ruegeria.
OX   NCBI_TaxID=292414;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TM1040;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Brettin T., Bruce D., Han C., Tapia R., Goodwin L., Thompson L.S.,
RA   Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA   Belas R., Moran M.A., Buchan A., Gonzalez J.M., Schell M.A., Sun F.,
RA   Richardson P.;
RT   "Complete sequence of chromosome of Silicibacter sp. TM1040.";
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000377; ABF65042.1; -; Genomic_DNA.
DR   RefSeq; WP_011539630.1; NC_008044.1.
DR   AlphaFoldDB; Q1GE74; -.
DR   SMR; Q1GE74; -.
DR   STRING; 292414.TM1040_2310; -.
DR   EnsemblBacteria; ABF65042; ABF65042; TM1040_2310.
DR   KEGG; sit:TM1040_2310; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000636; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..687
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101341"
SQ   SEQUENCE   687 AA;  74815 MW;  7201F24086EA4B75 CRC64;
     MADLLIELFS EEIPARMQAR AGEDLKKRMT DGLVEAGLTY AGAHALTTPR RLTLAIEGLL
     DHSPTVREER KGPKVGAPEK AIEGFLRGAG LTRDQLEERD TPKGAVYFAT IEKPGRPAAE
     IIAEVLEDTI RNFPWPKSMR WGAGALRWVR PLHSILCLLT REEGSEVVPL EIDGVTSGDS
     TAGHRFMAPA RFAVTGFEDY AAKLKRAFVV LDPKEREAAI WQDATNQAFA SGLEVVDDKG
     LLAEVAGLVE WPVVLMGSID AEFLDLPPEV LQTSMKEHQK FFSVKNPKTG RIEKFITVAN
     RETADNGATI LAGNQKVLSA RLADAKFFWE NDLRIAKSEV GVEGWLESLG NVTFHNKLGT
     QAARIDRIAA LAREIAPVVG ADADLAEQAA KVAKADLSSE MVYEFPELQG LMGRYYAQAA
     GLPQEVANAC ELHYSPLGPS DDVPTEPVSV AVALADKIDT LTGFWAIDEK PTGSKDPYAL
     RRAALGVIRL VLSNDVRVGL KEVFAKAYEG ADGSDLLSFF HDRLKVFLRD QGIRHDVIDA
     CIAMEGSDDL TLLVKRARAL EDFMKSEDGE NLLQGFKRAN NILSQAEEKD GVEYSFGADK
     KFVEDPAEAA LFEALAANEA AISTAIEAED FAAAMGGMAA LRAPVDAFFE AVQVNSDNPV
     VRRNRLNLLS QIRKVCGQVA DLSRIEG