ID   SYGB_SHEAM              Reviewed;         689 AA.
AC   A1S1I1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Sama_0025;
OS   Shewanella amazonensis (strain ATCC BAA-1098 / SB2B).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=326297;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1098 / SB2B;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J.,
RA   Richardson P.;
RT   "Complete sequence of Shewanella amazonensis SB2B.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000507; ABL98237.1; -; Genomic_DNA.
DR   RefSeq; WP_011758148.1; NC_008700.1.
DR   AlphaFoldDB; A1S1I1; -.
DR   SMR; A1S1I1; -.
DR   STRING; 326297.Sama_0025; -.
DR   EnsemblBacteria; ABL98237; ABL98237; Sama_0025.
DR   KEGG; saz:Sama_0025; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000009175; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..689
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006399"
SQ   SEQUENCE   689 AA;  74756 MW;  E538D2B8250B9E67 CRC64;
     MKFENLLIEI GTEELPPKAL RTLAESFQAN FSEELTKAEL PFESIEWYAA PRRLALYVKG
     LATAQEDKVV EKRGPAVQSA FDADGNPTKA AEGWARGNGI TVAEAERLAT DKGEWLVYRA
     NVAGEPTSVL VPAMTQRALD KLPIPKPMRW GSNSTQFIRP VHTVTLLLGS ELIAGEVLGI
     QSDRTIRGHR FMGESSFSLD HADNYLAALK ERGKVIADYQ ARKALIKADA EKAAAVIGGN
     ADIEESLLEE VASLVEWPVV LTAHFEEKFL AVPAEALVYT MKGDQKYFPV FSNDGKLLPN
     FIFVANIESK DPAQIIAGNE KVVRPRLADA EFFFNTDKKH TLASRLASLE TVVFQKQLGT
     LKDRAERISA LAGFIAGQIG ASSEDAARAG LLSKCDLMTN MVMEFTDTQG TMGMHYARLD
     GEAEAVAVAL EEQYKPKFSG DSVPSAGVSC AVALAEKLDT LSGIFGIGQA PKGAADPFAL
     RRAAIGVLRI IVENKLPLDL VDLIAKAVEL HGSNLSNALA ADEVLEFLMG RFRAWYQDKG
     ISTDVILAVL ARRPTRPADF DDRILAVSHF RTLEQAAALA AANKRVSNIL AKVEGELPSS
     INDALLSEAA EKALAAKLAE VTPVIAPLFA AGNYQQALTE LAALRESVDQ FFEDVMVMAD
     DQALRNNRLA LLGNLRDQFL HVADISLLQ