ID   SYGB_SHEDO              Reviewed;         694 AA.
AC   Q12TC1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Sden_0008;
OS   Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=318161;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OS217 / ATCC BAA-1090 / DSM 15013;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of Shewanella denitrificans OS217.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000302; ABE53305.1; -; Genomic_DNA.
DR   RefSeq; WP_011494474.1; NC_007954.1.
DR   AlphaFoldDB; Q12TC1; -.
DR   SMR; Q12TC1; -.
DR   STRING; 318161.Sden_0008; -.
DR   EnsemblBacteria; ABE53305; ABE53305; Sden_0008.
DR   KEGG; sdn:Sden_0008; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001982; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..694
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006402"
SQ   SEQUENCE   694 AA;  75591 MW;  8D27ADA4027976E0 CRC64;
     MKFENLLIEI GTEELPPKSL RTLADSFLAN FSDELVKADL PFTAATWYAA PRRLALSITG
     LAVAQADKVV EKRGPAVSSA FDADGNPTKA AQGWARGNGI TVEQAERLVT DKGEWLVHQA
     KVVGVQTNSL IAAMAQRALD KLPIPKPMRW GANSTQFIRP VHTVTMLLGS ELIGGELLGI
     KSDRIIRGHR FMGQASFQLD HADNYLSALK EQGKVLADYQ LRKAIIKADA EAAAAKIGGV
     ADIQEDLLEE VASLVEWPIV LTASFEEKFL NVPAEALVYT MKGDQKYFPV FDKSGKLLPN
     FIFVTNIESK DPQQIISGNE KVVRPRLADA EFFFNTDKKH SLASRLSSLE TVVFQKQLGT
     LKARAERISQ LAGFIATELN NNNLASSSVD AARAGLLSKA DLMTNMVMEF TDTQGTMGMH
     YARLDGETEA VAIAIEEQYK PKFSGDTVPT ASVSCAVALA EKLDTLVGIF GIGQAPKGAA
     DPFALRRAAI GILRIIVENK LPLDLVDLIA KAQALHGTNL TNGNTSEDVL EFLMARFRSW
     YQDKGIQVDV ILAVLARRPT RPADFDSRVN AVSHFRGLEA STALAAANKR VSNILAKVEG
     ELPANVTPSL LTETAEKALA EQLASLQPKL APLFATGDYQ QALTLLAELR ESVDQFFEDV
     MVMADDEALK NNRLALLNNL REQFLHVADI SLLQ