ID   SYGB_SHELP              Reviewed;         689 AA.
AC   A3Q8T4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-APR-2007, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Shew_0009;
OS   Shewanella loihica (strain ATCC BAA-1088 / PV-4).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=323850;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1088 / PV-4;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Serres G.,
RA   Fredrickson J., Tiedje J., Richardson P.;
RT   "Complete sequence of Shewanella loihica PV-4.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000606; ABO21882.1; -; Genomic_DNA.
DR   RefSeq; WP_011863819.1; NC_009092.1.
DR   AlphaFoldDB; A3Q8T4; -.
DR   SMR; A3Q8T4; -.
DR   STRING; 323850.Shew_0009; -.
DR   EnsemblBacteria; ABO21882; ABO21882; Shew_0009.
DR   KEGG; slo:Shew_0009; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001558; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..689
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006404"
SQ   SEQUENCE   689 AA;  75476 MW;  446F7D43D2D4A2A2 CRC64;
     MKFENLLIEV GTEELPPKSL RTLAESFLAN FEEELQKAEL SYESATWYAA PRRLALKVNA
     LVTAQADKVV EKRGPAIAQA FDADGNPTKA AMGWARGNGI TVEQAERLTT DKGEWLLHKA
     KVVGVETKSL VAEMAQRALD KLPIPKPMRW GSNKTQFIRP VHTVTMLMGD ELIPGELLGI
     ESARTIRGHR FMGVDTLELD HADNYLTALE TQGKVMADYQ ARMAIIKADA QAAADAMGGI
     AEIDEDLLEE VTSLVEWPVV LTANFEEKFL DVPAEALVYT MKGDQKYFPV FDKAGTLLPH
     FIFVSNIESK DPAQIIAGNE KVVRPRLADA EFFFETDKKH SLESRLASLE TVVFQQQLGT
     IKDRVERISA LAAFIAQGIG ADSEQAGRAG LLSKSDLMTN MVMEFTDLQG TMGMHYARLD
     GETEAVALAL EEQYKPKFSG DSVPTQPISI SVALAEKLDT LVGIFGIGQA PKGAADPFAL
     RRAAIGILRI CVENNLPLDL VDLVAKAQEL HGANLSNADT AEQVLEFLMG RFRAWYQDQG
     ISVDVILAVL ARRPTSPADF DSRIKAVTHF RSLEQAAALA AANKRVSNIL AKVEGDIASD
     IDSNLLQESA EQALASKLNE LQPQLEPLFA NADYQQALAL LASLRESVDT FFEDVMVMAD
     DEALKQNRLA LLSKLRAQFL HAADISLLQ