ID   SYGB_SHEPA              Reviewed;         689 AA.
AC   A8GYE8;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Spea_0006;
OS   Shewanella pealeana (strain ATCC 700345 / ANG-SQ1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=398579;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700345 / ANG-SQ1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C.,
RA   Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA   Zhao J.-S.Z., Manno D., Hawari J., Richardson P.;
RT   "Complete sequence of Shewanella pealeana ATCC 700345.";
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000851; ABV85335.1; -; Genomic_DNA.
DR   RefSeq; WP_012153283.1; NC_009901.1.
DR   AlphaFoldDB; A8GYE8; -.
DR   SMR; A8GYE8; -.
DR   STRING; 398579.Spea_0006; -.
DR   EnsemblBacteria; ABV85335; ABV85335; Spea_0006.
DR   KEGG; spl:Spea_0006; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000002608; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..689
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000078549"
SQ   SEQUENCE   689 AA;  75546 MW;  9A3672CC5C434430 CRC64;
     MNFENLLIEI GTEELPPKSL RKLAESFLAN FTEELTKAEL SFESAVWHAA PRRLAICINQ
     LALAQADKVV EKRGPAVAQA FDADGNPTKA AMGWARGNGI TVEQAERLKT DKGEWLLHQA
     RVVGVETKSL IADMAQRSLD KLPIPKPMRW GSNTTQFIRP VHTVTMLLGS EVVDGELLGI
     KSDRIIRGHR FMGESSFKLD HADNYLVALK EKGKVLADYE ARKAIIKTDA EAAAAKIGGV
     ADLEDDLLEE VTSLVEWPVV LTASFEEKFL DVPAEALVYT MKGDQKYFPV FDNAGQLLPN
     FIFVTNIESK DPQQIIAGNE KVVRPRLADA EFFFETDKKD TLEARLTSLE TVVFQKQLGT
     IKQRVERISA MAGYIATSID ANSEEAARAG LLSKSDLMTN MVMEFTDLQG TMGMHYARLN
     GETEAVAVAL SEQYKPKFSG DTVPTAPISI CVALAEKLDT LVGIFGIGQA PKGAADPFAL
     RRAAIGVLRI CLENNLPLDL VDLIAKAQEL HGENLTNADV AEQVLEFFMA RFRAWYQDQG
     VSVDVILAVL ARRPTAPADF ESRIKAVAHF RTLEQASALA AANKRVSNIL AKVEGELPAA
     IDDKLLVEAA EKALAEKLAE LQPQLAPLFA AANYQEALAL LASLRESVDT FFEDVMVMAD
     DEALKNNRLA LLSSLREQFL HAADISLLQ