ID   SYGB_SHESA              Reviewed;         688 AA.
AC   A0KR42;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=Shewana3_0016;
OS   Shewanella sp. (strain ANA-3).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=94122;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ANA-3;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Newman D.,
RA   Salticov C., Konstantinidis K., Klappenback J., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 1 of Shewanella sp. ANA-3.";
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000469; ABK46261.1; -; Genomic_DNA.
DR   RefSeq; WP_011715310.1; NC_008577.1.
DR   AlphaFoldDB; A0KR42; -.
DR   SMR; A0KR42; -.
DR   STRING; 94122.Shewana3_0016; -.
DR   EnsemblBacteria; ABK46261; ABK46261; Shewana3_0016.
DR   KEGG; shn:Shewana3_0016; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002589; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..688
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006407"
SQ   SEQUENCE   688 AA;  75154 MW;  5D292F87BFD7593C CRC64;
     MNFENLLIEL GTEELPPKAL RKLAESFLAN FTEELTKADL AFKSAVWYAA PRRLAINVTE
     LALAQADKIV EKRGPAVSSA FDAEGKPTKA AEGWARGNGI TVDQAERLVT DKGEWLVYNA
     KVEGVETKSL IAAMAQRALD KLPIPKPMRW GSSKTQFIRP VHTATMLLGS ELIEGELLGI
     KSARNVRGHR FMGTGFELDH ADNYLTLLKE KGKVIADYES RKALIKADAE KAAAKIGGTA
     DIEDDLLEEV TSLVEWPVVL TASFEEKFLN VPSEALVYTM KGDQKYFPVF DDAGKLLPNF
     IFVANIESKD PAQIIAGNEK VVRPRLADAE FFFNTDKKHT LESRLPSLET VLFQQQLGTL
     KDKVTRISAL AAFIAEQTGA NAVDAARAGL LSKTDLMTNM VMEFTDTQGT MGMHYARLDG
     ETEAVALAME EQYKPKFSGD TVPTAAVSCA VALADKLDTL VGIFGIGQAP KGAADPFALR
     RAAIGVLRII VENKLPLDLV TLIAKAQELH GTNLSNANAS DEVLEFLMAR FRAWYQDKGI
     EVDVILAVLA RRPTRPADFD SRINAVSHFR SLEASSALAA ANKRVSNILA KVEGELPTTI
     NSALLAEAAE QALAAKLAEL QPQLAPLFAN ADYQQALTLL ASLRESVDQF FEDVMVMADD
     EALKNNRLAL LNNLREQFLH VADISLLQ