ID   SYGB_SHESR              Reviewed;         688 AA.
AC   Q0I0U1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=Shewmr7_0008;
OS   Shewanella sp. (strain MR-7).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=60481;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MR-7;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nealson K.,
RA   Konstantinidis K., Klappenbach J., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 1 of Shewanella sp. MR-7.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000444; ABI41014.1; -; Genomic_DNA.
DR   RefSeq; WP_011624669.1; NC_008322.1.
DR   AlphaFoldDB; Q0I0U1; -.
DR   SMR; Q0I0U1; -.
DR   EnsemblBacteria; ABI41014; ABI41014; Shewmr7_0008.
DR   KEGG; shm:Shewmr7_0008; -.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..688
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006409"
SQ   SEQUENCE   688 AA;  75112 MW;  3DD7930F1C50BF4C CRC64;
     MNFENLLIEL GTEELPPKAL RKLAESFLAN FTEELTKADL AFKSAAWYAA PRRLAINVTE
     LAIAQADKIV EKRGPAVSSA FDAEGKPTKA AEGWARGNGI TVDQAERLVT DKGEWLVYNA
     KVEGVETKSL IAAMAQRALD KLPIPKPMRW GSSKTQFIRP VHTATMLLGS ELIEGELLGI
     KSARNVRGHR FMGTGFELDH ADNYLTLLKE KGKVIADYES RKALIKADAE KAAAKIGGTA
     DIEDDLLEEV TSLVEWPVVL TASFEEKFLN VPSEALVYTM KGDQKYFPVF DEAGKLLPNF
     IFVANIESKD PAQIIAGNEK VVRPRLADAE FFFNTDKKHT LESRLPSLET VLFQQQLGTL
     KDKVTRISAL AAFIAEQTGA NAVDAARAGL LSKTDLMTNM VMEFTDTQGT MGMHYARLDG
     ETEAVALAME EQYKPKFSGD TVPTAAVSCA VALADKLDTL VGIFGIGQAP KGAADPFALR
     RAAIGVLRII VENKLPLDLV TLIAKAQELH GTNLSNANAS DEVLEFLMAR FRAWYQDKGI
     DVDVILAVLA RRPTRPADFD SRINAVSHFR SLEASSALAA ANKRVSNILA KVEGELPTAI
     NSALLAEAAE QALAAKLAEL QPQLAPLFAN ADYQQALTLL SSLRESVDQF FEDVMVMADD
     EALKNNRLAL LNNLREQFLH VADISLLQ