ID   SYGB_SHEWM              Reviewed;         689 AA.
AC   B1KCY4;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Swoo_0012;
OS   Shewanella woodyi (strain ATCC 51908 / MS32).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=392500;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51908 / MS32;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Lykidis A., Zhao J.-S., Richardson P.;
RT   "Complete sequence of Shewanella woodyi ATCC 51908.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000961; ACA84313.1; -; Genomic_DNA.
DR   RefSeq; WP_012322662.1; NC_010506.1.
DR   AlphaFoldDB; B1KCY4; -.
DR   SMR; B1KCY4; -.
DR   STRING; 392500.Swoo_0012; -.
DR   EnsemblBacteria; ACA84313; ACA84313; Swoo_0012.
DR   KEGG; swd:Swoo_0012; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002168; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..689
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101339"
SQ   SEQUENCE   689 AA;  75751 MW;  665B937C11261BB4 CRC64;
     MNYENLLIEV GTEELPPKAL RKLAESFVSN FTDELEKAEL SFESAQWHAA PRRLAFTVNQ
     LVLAQADKVV EKRGPAIAQA FDADGNPTKA AMGWARGNGI SVEQAERLKT DKGEWLLHQA
     KVVGVETKSL INDMAQRALD KLPIPKPMRW GTSKTQFIRP VHTVTMLLGS ELVEGELLGV
     KSARVIRGHR FMGKKSFELD HADNYLSALK EQGMVLADYE ARKAIIKTDA EAAAAKIGGV
     ADLEDDLLEE VTSLVEWPVV LTANFEEKFL DVPAEALVYT MKGDQKYFPV FDKAGQLLPH
     FIFVTNIESK DPQVIISGNE KVVRPRLADA EFFFETDKKQ TLESRLASLE TVVFQKQLGT
     IKQRVERISQ LAGYIAASID ADSDEASRAG LLSKSDLMTN MVMEFTDLQG TMGMHYARLN
     GETEAVAVAL AEQYKPKFSG DTVPTAPISV CVALAEKLDT LVGIFGIGQA PKGAADPFAL
     RRAAIGVLRI CLENNLPLDL VDLIAKAQEL HGDNLTNDKA AEQVLEFFMG RFRAWYQDQG
     VSVDVILAVL ARRPTAPADF DSRIKAVAHF RSLEQASALA AANKRVSNIL AKVEGELATD
     ISSELLVENA EKALAEKLNE LQPQLAPLFA AANYQQALSL LADLRESVDT FFEDVMVMAD
     DEALKNNRLA LLSSLREQFL HAADISLLQ