ID   SYGB_SINMW              Reviewed;         721 AA.
AC   A6U6P3;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Smed_0466;
OS   Sinorhizobium medicae (strain WSM419) (Ensifer medicae).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=366394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WSM419;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G.,
RA   Richardson P.;
RT   "Complete sequence of Sinorhizobium medicae WSM419 chromosome.";
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000738; ABR59323.1; -; Genomic_DNA.
DR   RefSeq; WP_011974669.1; NC_009636.1.
DR   RefSeq; YP_001326158.1; NC_009636.1.
DR   AlphaFoldDB; A6U6P3; -.
DR   SMR; A6U6P3; -.
DR   STRING; 366394.Smed_0466; -.
DR   EnsemblBacteria; ABR59323; ABR59323; Smed_0466.
DR   GeneID; 61609740; -.
DR   KEGG; smd:Smed_0466; -.
DR   PATRIC; fig|366394.8.peg.3551; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001108; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 2.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..721
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101342"
SQ   SEQUENCE   721 AA;  78696 MW;  0C15F338144AAAB3 CRC64;
     MPDLLIELRS EEIPARMQRK AAGDLKKLVT DALVEAGLTY EGAREYWTPR RLTLDIRGLN
     ARSADMREER KGPRIDANDK AIEGFLRAAG LSSISEAHVH NDPKKGDFYV AHIVRRGREA
     EEIVADVMPA IIRDFPWPKS MRSGAASAIP GSLRWVRPLQ SIVCTFGAEH DETKVIPFEV
     DGIVASNITY GHRFHAPEAI AVRRFADYAE KLGKAKVVID AERRKEIISA DAHNIAFANG
     LELVEDEGLL EEVAGLVEWP KVLMGSFEES YLAIPSEIIR LTIKTNQKCF VTRKPGAETL
     SNRFILVSNI EAKDGGKEIV HGNGKVVRAR LSDAAHFWKR DQGDLPDLET LAASAGKFGL
     DLKKPLDQRM AKLDALNVTF HAKLGTQGER VARIRSLAAA LAKITGADPA SVARAAVLAK
     ADLRTEAVGE FPELQGIMGH KYAVLQGESA AVANAIEDHY KPQGPSDHVP TDPVSVSVAL
     ADKLDTLVGF WAIDERPTGS KDPYALRRAA LGVIRLILEG RARLPLLPCF DVALASLKLQ
     RPAMAADVSG DLLAFFHDRL KVYLRDLGAR HDLIDAVLAP DADDLLMIAR RVEALTAFIT
     AEEGRNLLAG TKRATQILAA EEKKGTEVAA SVDEELFRLD AERLLYASIK VASDGARAAI
     AKEDFRSAME ALSKLREPVD AFFNDVLVND EDKAIRANRL ALLGLIRSAT GEVADFSKIS
     G