ID   SYGB_STRA3              Reviewed;         679 AA.
AC   Q8CX30;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=gbs0260;
OS   Streptococcus agalactiae serotype III (strain NEM316).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=211110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEM316;
RX   PubMed=12354221; DOI=10.1046/j.1365-2958.2002.03126.x;
RA   Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T.,
RA   Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F.;
RT   "Genome sequence of Streptococcus agalactiae, a pathogen causing invasive
RT   neonatal disease.";
RL   Mol. Microbiol. 45:1499-1513(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AL766844; CAD45905.1; -; Genomic_DNA.
DR   RefSeq; WP_000159092.1; NC_004368.1.
DR   AlphaFoldDB; Q8CX30; -.
DR   SMR; Q8CX30; -.
DR   STRING; 211110.gbs0260; -.
DR   PRIDE; Q8CX30; -.
DR   EnsemblBacteria; CAD45905; CAD45905; CAD45905.
DR   KEGG; san:glyS; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000000823; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..679
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101344"
SQ   SEQUENCE   679 AA;  76610 MW;  A3E0A5C98EE0E6FE CRC64;
     MTKDLLLELG LEELPAYVVT PSEKQLGQKM VKFLEDHRLS FETVQTFSTP RRLAVRVKGL
     ADQQTDLTED FKGPSKKIAL DAEGNFSKAA QGFVRGKGLS VDDIEFREVK GEEYVYVTKH
     ETGKSAIDVL ASVTEVLTEL TFPVNMHWAN NSFEYIRPVH TLVVLLDDQA LELDFLDIHS
     GRISRGHRFL GSDTEILSAS SYEDDLRQQF VIADAKERQQ MIVDQIHAIE EKENISVEID
     EDLLNEVLNL VEYPTAFLGS FDEKYLDVPE EVLVTSMKNH QRYFVVRDRD GKLLPNFISV
     RNGNAEHIEN VIKGNEKVLV ARLEDGEFFW QEDQKLNIAD LVEKLKQVTF HEKIGSLYEH
     MDRVKVISQY LAEKADLSDE EKLAVLRAAS IYKFDLLTGM VDEFDELQGI MGEKYALLAG
     EQPAVAAAIR EHYMPTSADG ELPETRVGAI LALADKFDTL LSFFSVGLIP SGSNDPYALR
     RATQGIVRIL EAFGWDIPLD ELVTNLYGLS FASLDYANQK EVMAFISARI EKMIGSKVPK
     DIREAVLESD TYIVSLILEA SQALVQKSKD AQYKVSIESL SRAFNLAEKV THSVSVDYSL
     FENNQEKALY QAILSLELTE DMHDNLDKLF ALSPIINDFF DNTMVMTDDE KMKQNRLALL
     NSLVAKARTV AAFNLLNTK