ID   SYGB_SULSY              Reviewed;         678 AA.
AC   B2V9P9;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=SYO3AOP1_1053;
OS   Sulfurihydrogenibium sp. (strain YO3AOP1).
OC   Bacteria; Aquificae; Aquificales; Hydrogenothermaceae;
OC   Sulfurihydrogenibium; unclassified Sulfurihydrogenibium.
OX   NCBI_TaxID=436114;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YO3AOP1;
RX   PubMed=19136599; DOI=10.1128/jb.01645-08;
RA   Reysenbach A.-L., Hamamura N., Podar M., Griffiths E., Ferreira S.,
RA   Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A.,
RA   Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A.;
RT   "Complete and draft genome sequences of six members of the Aquificales.";
RL   J. Bacteriol. 191:1992-1993(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001080; ACD66672.1; -; Genomic_DNA.
DR   RefSeq; WP_012459740.1; NC_010730.1.
DR   AlphaFoldDB; B2V9P9; -.
DR   SMR; B2V9P9; -.
DR   STRING; 436114.SYO3AOP1_1053; -.
DR   EnsemblBacteria; ACD66672; ACD66672; SYO3AOP1_1053.
DR   KEGG; sul:SYO3AOP1_1053; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_0; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..678
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101362"
SQ   SEQUENCE   678 AA;  78884 MW;  65A29045FDBBCB5E CRC64;
     MKSYLLEIGC EELPPKAILT YKEFLKEYAH QTFKDFFIYN SPENIKIYAT PRRLAVLIKN
     LKKKQDNQKI TLIGPPYKVA VDSEGKFTKA ALSFAEKNNI PLEKLEKITT EKGEYLGATI
     EKEGESLELF IKHKIPQLFN QFPQLKSMKW NNSDYRFPRP IRWIVSLLDD KVIEFEVASV
     KTDRFTHLHR FMTKPIGRGE RKDINHANDY EEITKLGYII ANFEDRKHSI KTQYEGFARQ
     LNASIIEDDE LIDEITCLTE FPVGIVGDFS PEYLTLPKEV IITVCKHHQR YLNFEKDGKL
     IPKFLAFSNN AVKDRDIVKN GYEKVLRARL EDALFFYKED LKKKLDDNIE KLKGIQFHEK
     LGSMYDKVLR NLELALKLAD LTGYKDAEKI KRAVMLSKAD LLTEMVKEFD ELQGIMGMYY
     SQKQGEEEEI SKSIYEHYLP KTAEDNIPET NLGTLLALAD KLDTVISFIK IGELPKPSAD
     PFGIRRNAIG IVRLLVEKEI DLDLRKVIDD ESILDFILSR LESYLQSKGY KTDIINAVLS
     LKDGNIYRNY LKVKALSQLR NLPDYENVIM VFKRVGNIIP EDFKFSNVDV NLLVSQPEKE
     LYKKFIEIKD KFKRFIENKD YDKALGLLLE LKPYIDRFFD NVMIMVEDEK LKNNRLSLLK
     EINDLFRNIA DFTKLIGG