ID   SYGB_SYNAS              Reviewed;         690 AA.
AC   Q2LVI9;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=SYNAS_22160;
GN   ORFNames=SYN_01537;
OS   Syntrophus aciditrophicus (strain SB).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Syntrophales; Syntrophaceae;
OC   Syntrophus.
OX   NCBI_TaxID=56780;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SB;
RX   PubMed=17442750; DOI=10.1073/pnas.0610456104;
RA   McInerney M.J., Rohlin L., Mouttaki H., Kim U., Krupp R.S.,
RA   Rios-Hernandez L., Sieber J., Struchtemeyer C.G., Bhattacharyya A.,
RA   Campbell J.W., Gunsalus R.P.;
RT   "The genome of Syntrophus aciditrophicus: life at the thermodynamic limit
RT   of microbial growth.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:7600-7605(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000252; ABC78095.1; -; Genomic_DNA.
DR   RefSeq; WP_011418115.1; NC_007759.1.
DR   AlphaFoldDB; Q2LVI9; -.
DR   SMR; Q2LVI9; -.
DR   STRING; 56780.SYN_01537; -.
DR   PRIDE; Q2LVI9; -.
DR   EnsemblBacteria; ABC78095; ABC78095; SYN_01537.
DR   KEGG; sat:SYN_01537; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_7; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001933; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..690
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006417"
SQ   SEQUENCE   690 AA;  77603 MW;  C33A34B4D1DBD80F CRC64;
     MSNELLLEIG TEEIPAAFLP KALQDMSSMI RKALTEARIP FGQVHTFGTP RRLCLAVADV
     AEKQEDQVIE KLGPARRVSF DADGNPTKAA LGFAKSQGVD ISEIGTMQTD KGEYICISRH
     ISGKSTVSLL SEMLSRLITS LSFKKSMRWG NLDFRFARPI HWILALYGGE VIPFRIENIE
     SGATSRGHRF MHPEAFPVSN LQEYLARTRE HFVIVAPEER KRIILEEARK AAAAVSGRVL
     ENEDLLETVT YLVEYPTIVC GSFDRKYLEL PKEVLITSMM SHQKYFPVVD QEGRLLPFFI
     TINNTLARDP AVVTRGNEKV IRARLSDAQF FFEEDQKIRL DDRVEGLQQV VFHTLLGTSY
     EKVQRFRKLA GWIADRIDPS LKNRVNRSAL LAKADLDTQM VGEFSELQGI MGREYALLAG
     EDPTVARAIY EHYLPLTAGG DLPQTHEGAI VSIADKMDSI AGFFGVNLVP TGTADPYALR
     RQALGVINII LDKKYPLTLD DLVDECISIL EEKLKRPAEE TRKDVIEFFR GRLENMLISQ
     GHPHDVVSAV LAAGFADLVQ VIKKIEAMES FKAHPAYEPL AIAFKRAGNI LKEFRNGRID
     PALFSAAEEN QLYSTLLEAR ARVVKALEKD DYPAALLELA ALRQPIDHFF ESVMVMVDEE
     NIRFNRLSLL EALFSIFRRI ADFSRIVTES