ID   SYGB_THENN              Reviewed;         670 AA.
AC   B9K6R1;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=CTN_0468;
OS   Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=309803;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E;
RA   Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J.,
RA   Kim J.J., Park K.J., Lee S.Y.;
RT   "The genome sequence of the hyperthermophilic bacterium Thermotoga
RT   neapolitana.";
RL   Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000916; ACM22644.1; -; Genomic_DNA.
DR   RefSeq; WP_015918963.1; NC_011978.1.
DR   AlphaFoldDB; B9K6R1; -.
DR   SMR; B9K6R1; -.
DR   STRING; 309803.CTN_0468; -.
DR   EnsemblBacteria; ACM22644; ACM22644; CTN_0468.
DR   KEGG; tna:CTN_0468; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_0; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000000445; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..670
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197224"
SQ   SEQUENCE   670 AA;  77492 MW;  AD5D64F5EE43C1DA CRC64;
     MRTALLEVGL EELPASEFYN ILEQLETRTR ELLKANRIQC DSIEVFVGSR RFGVLLRGLP
     EKQEGFVEEK KGPPLNVAYD SKGAPTRALE GFLRKNNATF EDIIEKDGYV YISRKIEGKA
     VEEVLPEVFE DLVMGMSFKK PMRWGTGEYE YVRPVHWIVA MLDDRVLNLT LFGLRASHVS
     YGKRYHSGAL KINTPEEYYE VLKSGYVMAS HEERKKSVLK QLEDFERTYG MKVERDEALI
     AEIVAMTEYP RILVGQFDQK YLELPEEIIV TAVKHHQRAF VAHKDGLTNF FVAFQDGPQS
     SENVVKGYER VINARLEDAR YYFHKDLEMS LGEMNERLKG IVFQERLGTL YDKVERIVKI
     SRKICGELKF PEDFTEKVLK VASLCKADIA SKVVYEFPEL QGVMGRIYAL KEGMDEELAF
     AIEDHYSENP ETVIGSVLGM ADRLDTIVGN FAIGNIPTSS KDPYGLKGKA DTVFRIVRKN
     EWDVSLEELL NFAASLVGFH LSNDLEEFFS SRFYQFLINE IGVSYDVARA VNHLWKKPLR
     GTLAAEALQN ISERPEFQDL FVGFERVHNI TKKHDSREFD GALFEKEEEK RLMNKFFEVK
     EKVLKALERL NYEEALQYLI ELKPYIDEYF DNVFVMVNRD DLRMNRLGFL KNIDDLFMMI
     GDMSHLVKRT