ID   SYGB_THIDA              Reviewed;         701 AA.
AC   Q3SGF0;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Tbd_2347;
OS   Thiobacillus denitrificans (strain ATCC 25259).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Thiobacillaceae; Thiobacillus.
OX   NCBI_TaxID=292415;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25259;
RX   PubMed=16452431; DOI=10.1128/jb.188.4.1473-1488.2006;
RA   Beller H.R., Chain P.S., Letain T.E., Chakicherla A., Larimer F.W.,
RA   Richardson P.M., Coleman M.A., Wood A.P., Kelly D.P.;
RT   "The genome sequence of the obligately chemolithoautotrophic, facultatively
RT   anaerobic bacterium Thiobacillus denitrificans.";
RL   J. Bacteriol. 188:1473-1488(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000116; AAZ98300.1; -; Genomic_DNA.
DR   RefSeq; WP_011312859.1; NC_007404.1.
DR   AlphaFoldDB; Q3SGF0; -.
DR   SMR; Q3SGF0; -.
DR   STRING; 292415.Tbd_2347; -.
DR   EnsemblBacteria; AAZ98300; AAZ98300; Tbd_2347.
DR   KEGG; tbd:Tbd_2347; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000008291; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..701
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101367"
SQ   SEQUENCE   701 AA;  74976 MW;  7AB01C1B158AD320 CRC64;
     MTTANLLVEL FVEELPPKAL KKLGEAFAAA LTESLVAQGL VHAQAAVTHF ASPRRLGAHI
     ENVLERSADK PVSTKLMPVS VGLDADGNAT PALLKRLAAL GADASAVATL RRESDGKAEA
     LFMDSVAKGI SLGLGLAKAL WESLDKLPIP KVMSYQLADG WSTVNFVRPV HGLVALHGTD
     PVDVRMFGLT AGRKTHGHRF EAAADPIVLK DADSYAQQLE SEGAVIANFA ERRAEIVRQL
     EAAAAPLGLV PVEDEALLDE VTALVERPNV LLGQFEQAFL EVPQECLILT MKANQKYFPL
     LDAAGKLTNK FLIVSNISPA DASAVVGGNE RVVRPRLADA KFFFDQDRKK TLDARVLGLA
     KVVYHNKLGT QGERVTRVQA VARAIGEKLG GEALALKADQ AALLAKADLL TDMVGEFPEL
     QGVMGRYYAQ HDGVADDVAF AIEDHYRPRF AGDDLPRNPV GVCVALADKL ETLVGLFGIG
     EKPTGDKDPF ALRRHALGVV RMLIEKHLPI GLNELVSIAF SAFQQGLLGQ AHTDVYLFMF
     ERLSGAMREQ GYSANEVDAV LSLNPIRIDL VPQQLEAVRA FAALPEAAAL AAANKRVGNI
     LKKAEGEVGA SADPGRFVEA AERALFAALD EVAPRAAAAF GSGDYTASLQ ALAALKAPVD
     AFFDQVMVNA DDPALRANRL ALLNQLHRTM NRVADISRLA A