ID   SYGB_VIBA3              Reviewed;         688 AA.
AC   B7VGK6;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=VS_0032;
OS   Vibrio atlanticus (strain LGP32) (Vibrio splendidus (strain Mel32)).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=575788;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LGP32;
RA   Mazel D., Le Roux F.;
RT   "Vibrio splendidus str. LGP32 complete genome.";
RL   Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FM954972; CAV17089.1; -; Genomic_DNA.
DR   RefSeq; WP_012602954.1; NC_011753.2.
DR   AlphaFoldDB; B7VGK6; -.
DR   SMR; B7VGK6; -.
DR   STRING; 575788.VS_0032; -.
DR   EnsemblBacteria; CAV17089; CAV17089; VS_0032.
DR   KEGG; vsp:VS_0032; -.
DR   PATRIC; fig|575788.5.peg.1446; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000009100; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..688
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197226"
SQ   SEQUENCE   688 AA;  75785 MW;  AE343A7BBC09DB24 CRC64;
     MAKNFLIELG TEELPPTALR SLAEAFASNF EAGLKTAELS HEGIKWYAAP RRLALKVTAL
     AEGQADKVVE KRGPAISVAF DAEGNATKAA QGWARGNGIT VEQADRLKTD KGEWLLFKQE
     VAGKPVQELV MDIAAKALAG LPIPKAMRWG NSDIQFIRPV KTLTVLLGDE LVEGKILGVA
     SARTIRGHRF MGEQEFTIDS ADQYPAILEE RGKVMADYDA RKAIILADAK KAADAVGGIA
     DLEDDLVEEV TSLVEWPVVL TAKFEQVFLK VPSEALVYTM KGDQKYFPVY DADKNLLPNF
     IFVSNIESKE PRHVIEGNEK VVRPRLADAE FFFNTDRKRP LIDRLAELDQ AIFQKQLGTI
     KDKTDRITEL AGYIAEQIDA DVEKSKRAGL LAKCDLMTSM VFEFTDTQGV MGMHYATHDG
     EDEQVALALY EQYMPRFAGD TLPSTGISSA VAMADKLDTI VGIFGIGQAP KGSDPFALRR
     ASLGVLRIIV ENGYNLDLTD LIGKAKELLG DKLTNENVEA DVIDFMLGRF RAWYQDAGFS
     VDIIQAVLAR RPTKPADFDQ RVKAVSHFRE LEAAEALAAA NKRVGNILAK FDGELPAEID
     LALLQEDAEK ALAENVEVMT EALEPAFATG NYQEALSKLA DLREPVDAFF DNVMVMADDE
     ALKKNRLTLL NNLRNLFLQI ADISLLQK