ID   SYGB_VIBC1              Reviewed;         693 AA.
AC   A7N1D8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=VIBHAR_00449;
OS   Vibrio campbellii (strain ATCC BAA-1116).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=2902295;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1116 / BB120;
RG   The Vibrio harveyi Genome Sequencing Project;
RA   Bassler B., Clifton S.W., Fulton L., Delehaunty K., Fronick C.,
RA   Harrison M., Markivic C., Fulton R., Tin-Wollam A.-M., Shah N., Pepin K.,
RA   Nash W., Thiruvilangam P., Bhonagiri V., Waters C., Tu K.C., Irgon J.,
RA   Wilson R.K.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000789; ABU69464.1; -; Genomic_DNA.
DR   RefSeq; WP_012126670.1; NC_022269.1.
DR   AlphaFoldDB; A7N1D8; -.
DR   SMR; A7N1D8; -.
DR   EnsemblBacteria; ABU69464; ABU69464; VIBHAR_00449.
DR   KEGG; vha:VIBHAR_00449; -.
DR   PATRIC; fig|338187.25.peg.2141; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000008152; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..693
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006420"
SQ   SEQUENCE   693 AA;  76679 MW;  338684454AF34EDC CRC64;
     MAKEFLIELG TEELPPTQLR TLAEAFAANF EAELKGAELA HEGVKWFAAP RRLALKVAAL
     ADSQSDKVVE KRGPAVSAAF DAEGNPTKAA QGWARGCGIT VDQAERMVTD KGEWLLFKQE
     VKGQPTSQIV VELAAKALAN LPIAKPMRWG NKTTQFIRPV KTLTMLMGSD LIEGEILGVA
     SDRTIRGHRF MGEQEFTIDS AEQYPAILEE RGKVMADYEA RKAIILADAQ KAAAAVGGIA
     DLEDDLVEEV TSLVEWPVVL TAKFEEEFLK VPSEALVYTM KGDQKYFPVY SHENGDENKK
     LLPNFIFVSN IESKEPRYVI EGNEKVVRPR LADAEFFFNT DRKRPLIDRL PELEQAIFQK
     QLGTIKDKTD RITELAGYIA EQIGADVEKS KRAGLLAKCD LMTSMVFEFT DTQGVMGMHY
     ARHDGEAEEV AVALNEQYMP RFAGDELPSN GVSTAVAMAD KLDTIVGIFG IGQAPKGSDP
     FALRRASLGV LRIIVEYGYN LDLVDLVAKA KSLFGDRLTN DNVEQDVIEF MLGRFRAWYQ
     DEGFSVDIIQ AVLARRPTKP ADFDQRVKAV SHFRELEAAE SLAAANKRVG NILAKFDGEL
     AADIDLALLQ EDAEKALAES VEVMTEALEP AFATGNYQEA LSKLADLREP VDAFFDNVMV
     MADDEALKKN RLTLLNNLRN LFLQIADISL LQK