ID   SYGB_VIBPA              Reviewed;         688 AA.
AC   Q87TP8;
DT   16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=VP0021;
OS   Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=223926;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RIMD 2210633;
RX   PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA   Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA   Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA   Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT   "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT   distinct from that of V. cholerae.";
RL   Lancet 361:743-749(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; BA000031; BAC58284.1; -; Genomic_DNA.
DR   RefSeq; NP_796400.1; NC_004603.1.
DR   RefSeq; WP_005458686.1; NC_004603.1.
DR   AlphaFoldDB; Q87TP8; -.
DR   SMR; Q87TP8; -.
DR   STRING; 223926.28805003; -.
DR   EnsemblBacteria; BAC58284; BAC58284; BAC58284.
DR   GeneID; 1187477; -.
DR   KEGG; vpa:VP0021; -.
DR   PATRIC; fig|223926.6.peg.21; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000002493; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..688
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_0000072936"
SQ   SEQUENCE   688 AA;  76199 MW;  E2E9F24400FE1D5B CRC64;
     MAKEFLIELG TEELPPTQLR TLAEAFAANF EAELKGAELA HEGVKWFAAP RRLALKVAAL
     AESQSDKVVE KRGPAVSAAF DAEGNPTKAA QGWARGCGIT VDQADRMVTD KGEWLLFKQE
     VKGQPTSEIV VELAAKALAN LPIAKPMRWG NKTTQFIRPV KTLTMLMGSD LIEGEILGVA
     SSRTIRGHRF MGEKEFTIDS AEQYPAILEE RGKVMADYEA RKAIILADAQ KAAAAVGGIA
     DLEDDLVEEV TSLVEWPVVL TAKFEEEFLK VPSEALVYTM KGDQKYFPVY DENKKLLPNF
     IFVSNIESKE PRYVIEGNEK VVRPRLADAE FFFNTDRKRP LIDRLPELEQ AIFQQQLGTI
     KDKTDRITEL AGYIAEQIGA DVEKSKRAGL LAKCDLMTSM VFEFTDTQGV MGMHYARHDG
     EAEEVAVALN EQYMPRFAGD ELPSNGVSTA VAMADKLDTI VGIFGIGQAP KGSDPFALRR
     ASLGVLRIIV EYGYNLDLVD LVAKAKSLFG DRLTNDNVEQ DVIEFMLGRF RAWYQDEGFS
     VDIIQAVLAR RPTKPADFDQ RVKAVSHFRE LEAAESLAAA NKRVGNILAK FDGELAEEID
     LALLQEDAEK ALAESVEVMT EALEPAFATG NYQEALSKLA DLREPVDAFF DNVMVMADDE
     ALKKNRLTLL NNLRNLFLQI ADISLLQK